Presentation is loading. Please wait.

Presentation is loading. Please wait.

Origin of endothelial cells that line expanded polytetrafluoroethylene vascular grafts sodded with cells from microvascularized fat  Stuart K. Williams,

Published byVictoria Richardson Modified over 6 years ago

Similar presentations

Presentation on theme: "Origin of endothelial cells that line expanded polytetrafluoroethylene vascular grafts sodded with cells from microvascularized fat  Stuart K. Williams,"— Presentation transcript:

1 Origin of endothelial cells that line expanded polytetrafluoroethylene vascular grafts sodded with cells from microvascularized fat  Stuart K. Williams, PhD, Leigh B. Kleinert, AS, Deborah Rose, BS, Sandra McKenney, BS, *  Journal of Vascular Surgery  Volume 19, Issue 4, Pages (April 1994) DOI: /S (94) Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

2 Fig. 1 Immunofluorescent staining of falciform ligamental fat cryostat-cut sections after reaction with anti-vWF antibodies (a), with nuclear staining of same cryostat-cut section (b); or after reaction with anticytokeratin peptide 18 antibodies (c), with nuclear staining of same cryostat-cut section (d). Bar, 12 μm. Journal of Vascular Surgery  , DOI: ( /S (94) ) Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

3 Fig. 2 Fluorescence emission from PKH-26–labeled, freshly isolated canine falciform ligamental cells plated on tissue culture plastic. Both attached (arrow) and round (arrowhead) cells emit fluorescence. Bar, 36 μm. Journal of Vascular Surgery  , DOI: ( /S (94) ) Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

4 Fig. 3 Growth curve of canine falciform ligamental cells. Cells were labeled with PKH-26 at a final dye concentration of 2 μmol/L (○), 5 μmol/L (▵); or without fluorescent label (□). Journal of Vascular Surgery  , DOI: ( /S (94) ) Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

5 Fig. 4 Fluorescent micrograph of fluorescently labeled sodded graft before implantation. a, En face observation of the flow surface; bar, 36 mm. b, Cross section of the sodded graft; bar, 36 μm. Journal of Vascular Surgery  , DOI: ( /S (94) ) Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

6 Fig. 5 Morphologic characteristics of the PKH-26 labeled sodded grafts at the time of explantation. a, Scanning-electron micrograph of the midgraft flow surface; bar, 15 μm. b, Trichrome-stained section of the midgraft region; bar, 28 μm. Journal of Vascular Surgery  , DOI: ( /S (94) ) Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

7 Fig. 6 Fluorescence emission from the flow surface of sodded grafts at the time of explantation. Surfaces were evaluated with a rhodamine filter set. a, En face view of the flow surface; bar, 62 μm. b, Cross section of the flow surface; bar, 36 μm. Journal of Vascular Surgery  , DOI: ( /S (94) ) Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

8 Fig. 7 a, Cross section of a sodded graft stained with antibody to vWF. Fluorescein isothiocyanate excitation and emission filter block; bar, 36 μm. b, Cross section of a segment of sodded graft incubated with DiI-ac-LDL. Rhodamine excitation and emission filter block; bar, 36 μm. Journal of Vascular Surgery  , DOI: ( /S (94) ) Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Download ppt "Origin of endothelial cells that line expanded polytetrafluoroethylene vascular grafts sodded with cells from microvascularized fat  Stuart K. Williams,"

Similar presentations

Endothelial cell seeding reduces thrombogenicity of Dacron grafts in humans Per Örtenwall, MD, PhD *, Hans Wadenvik, MD, PhD **, Jack Kutti, MD, PhD **,

Endothelial cell seeding reduces thrombogenicity of Dacron grafts in humans Per Örtenwall, MD, PhD *, Hans Wadenvik, MD, PhD **, Jack Kutti, MD, PhD **,
The effect of inguinal lymphatic manipulation on regional lymph flow patterns Jeffrey R. Rubin, MD, Lisa B. Eberlin, MD Journal of Vascular Surgery Volume.

The effect of inguinal lymphatic manipulation on regional lymph flow patterns Jeffrey R. Rubin, MD, Lisa B. Eberlin, MD Journal of Vascular Surgery Volume.
Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold 

Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold 
Do patients with the heparin-induced thrombocytopenia syndrome have heparin-specific antibodies?  John G. Adams, MD, Loren J. Humphrey, MD, PhD, Xinchao.

Do patients with the heparin-induced thrombocytopenia syndrome have heparin-specific antibodies?  John G. Adams, MD, Loren J. Humphrey, MD, PhD, Xinchao.
Liposuction-derived human fat used for vascular graft sodding contains endothelial cells and not mesothelial cells as the major cell type  Stuart K. Williams,

Liposuction-derived human fat used for vascular graft sodding contains endothelial cells and not mesothelial cells as the major cell type  Stuart K. Williams,
Robert J. Pitsch, MD, David J. Minion, MD, Margaret L

Robert J. Pitsch, MD, David J. Minion, MD, Margaret L
Folic acid inhibits homocysteine-induced proliferation of human arterial smooth muscle cells  Brennan J. Carmody, MD, Subodh Arora, MD, Ricardo Avena,

Folic acid inhibits homocysteine-induced proliferation of human arterial smooth muscle cells  Brennan J. Carmody, MD, Subodh Arora, MD, Ricardo Avena,
Infection of vascular prostheses caused by bacterial biofilms

Infection of vascular prostheses caused by bacterial biofilms
Development of a “leak-proof,” knitted Dacron vascular prosthesis

Development of a “leak-proof,” knitted Dacron vascular prosthesis
Decellularization reduces immunogenicity of sheep pulmonary artery vascular patches  Eric J. Lehr, MD, PhD, Gina R. Rayat, PhD, Brian Chiu, MD, Thomas.

Decellularization reduces immunogenicity of sheep pulmonary artery vascular patches  Eric J. Lehr, MD, PhD, Gina R. Rayat, PhD, Brian Chiu, MD, Thomas.
Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold 

Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold 
Somatostatin gene transfer and expression in endothelial cells

Somatostatin gene transfer and expression in endothelial cells
Karen M. Newman, PhD, Jessie Jean-Claude, MD, Hong Li, MS, John V

Karen M. Newman, PhD, Jessie Jean-Claude, MD, Hong Li, MS, John V
Endothelial sodding of the permaflow prosthetic coronary artery bypass conduit  Michael R Phillips, MD, Hiroki Yamaguchi, MD, Virginia M Miller, PhD, Stuart.

Endothelial sodding of the permaflow prosthetic coronary artery bypass conduit  Michael R Phillips, MD, Hiroki Yamaguchi, MD, Virginia M Miller, PhD, Stuart.
The role of circulating cells in the healing of vascular prostheses

The role of circulating cells in the healing of vascular prostheses
A new valvulotome for in situ bypass grafts

A new valvulotome for in situ bypass grafts
Nicholas P. Ziats, PhD, James M. Anderson, MD, PhD 

Nicholas P. Ziats, PhD, James M. Anderson, MD, PhD 
Long-term results of tissue development and cell differentiation on dacron prostheses seeded with microvascular cells in dogs  Gabriela Baitella-Eberle,

Long-term results of tissue development and cell differentiation on dacron prostheses seeded with microvascular cells in dogs  Gabriela Baitella-Eberle,
D. A. Lewis, PhD, R. C. Lowell, MD, R. A. Cambria, MD, P. C

D. A. Lewis, PhD, R. C. Lowell, MD, R. A. Cambria, MD, P. C
Alex Westerband, MD, Joseph L. Mills, MD, John M. Marek, MD, Ronald L

Alex Westerband, MD, Joseph L. Mills, MD, John M. Marek, MD, Ronald L

Similar presentations

Ads by Google