1. 6/27/20111 NEW YORK MEDICAL COLLEGE Environmental Health and Safety Department LABORATORY SAFETY Good Laboratory Practices Training Program

2. 6/27/20112 Objectives Apply the recommended guidelines for personal laboratory safetyApply the recommended guidelines for personal laboratory safety Identify procedures when spills occursIdentify procedures when spills occurs Identify differences between different categories of wasteIdentify differences between different categories of waste Identify and apply proper procedures for recyclingIdentify and apply proper procedures for recycling

3. 6/27/20113 Rules and Regulations OSHA=Occupational Safety and Health AdministrationOSHA=Occupational Safety and Health Administration EPA= Environmental Protection AgencyEPA= Environmental Protection Agency NIH=National Institute of HealthNIH=National Institute of Health CDC= Center for Disease ControlCDC= Center for Disease Control DEC= Department of Environmental ConservationDEC= Department of Environmental Conservation NYSDOH= New York State Department of HealthNYSDOH= New York State Department of Health DOT= Department of TransportationDOT= Department of Transportation NYMC =Lab Safety ManualNYMC =Lab Safety Manual

4. 6/27/20114 Proper Attire When working in the lab you MUST:When working in the lab you MUST: –Wear lab coat –Safety glasses (eye protection) –Gloves When working in the lab NEVER wear:When working in the lab NEVER wear: –Shorts –Open-toed shoes –Tank-tops

5. 6/27/20115 At New York Medical College Children and Pets are not allow in laboratoriesChildren and Pets are not allow in laboratories Security Dept enforces this policySecurity Dept enforces this policy

6. 6/27/20116 Use mechanical/electronic devices for pipetting NEVER

7. 6/27/20117 Be aware of: Dangling jewelryDangling jewelry Loose clothing andLoose clothing and Long hairLong hair As they might get caught in equipment

8. 6/27/20118 Use designated areas for eating and drinking Do not wear lab coat in the cafeteria…!!!

9. 6/27/20119 Do not wear lab coats, gloves or other personal protective clothing out of the lab and into non-lab areas If gloves are necessary, have a glove in one hand only

10. 6/27/201110 Wash your hands frequently throughout the day and before leaving the lab.

11. 6/27/201111 Know where the eye wash and safety shower is in your department areaKnow where the eye wash and safety shower is in your department area In case of splash in your eyes, flush eyes with tap water for 15 minutes with eyewash

12. 6/27/201112 Store foods and drinks only in designated refrigerators…… Food only Reagents only

13. 6/27/201113 Cleaning and decontamination Clean your work area throughout the day and before you leave at the end of the day.Clean your work area throughout the day and before you leave at the end of the day. If necessary, clean equipment after use to avoid the possibility of contaminating the next person who needs to use it.If necessary, clean equipment after use to avoid the possibility of contaminating the next person who needs to use it. Keep all aisles and walkways in the lab clear to provide a safe walking surface and unobstructed exit ways.Keep all aisles and walkways in the lab clear to provide a safe walking surface and unobstructed exit ways.

14. 6/27/201114 Non-hazardous waste Non-hazardous waste –Paper/Cardboard (recyclable) –Food containers (cleaned can be recycled) –Empty Pipette Tip boxes, Microcentrifuge tubes, conical tubes, (recyclable) –Petri dishes used with non-infectious agents after cleaned (can be recycled) –Gloves not contaminated with hazardous agents go in regular garbage non-recyclable) –Empty chemical containers, glass/plastic, (deface label, rinse 3x with water=recyclable)

15. 6/27/201115 Hazardous waste: Biological Biological: DRY waste that belongs in Red BagsBiological: DRY waste that belongs in Red Bags Human pathological wasteHuman pathological waste Other Infectious wasteOther Infectious waste Biological LIQUID Waste: Infectious Cultures and stocks must be treated overnight with a freshly made 10%Bleach Solution and then discarded into the sink (questions? Call EHS @ 594-4078)Biological LIQUID Waste: Infectious Cultures and stocks must be treated overnight with a freshly made 10%Bleach Solution and then discarded into the sink (questions? Call EHS @ 594-4078)

16. 6/27/201116 Biological Spill In case of biological hazard spill use the emergency kit located inside the Chemical Spill Kit white bucket. Read carefully and follow instructions on orange label, then dispose inside a red bag containerIn case of biological hazard spill use the emergency kit located inside the Chemical Spill Kit white bucket. Read carefully and follow instructions on orange label, then dispose inside a red bag container

17. 6/27/201117 Biological waste disposal Grey containers (32gal) in hallwaysGrey containers (32gal) in hallways Red containers lined with red bags in labsRed containers lined with red bags in labs Freezers in Animal Facility (dead animals)Freezers in Animal Facility (dead animals) Sharps containers: grey/red (17gal),Sharps containers: grey/red (17gal), : Dead animals SHOULD NOT be kept in lab freezersNote: Dead animals SHOULD NOT be kept in lab freezers

18. Chemical Inventory All hazardous chemical substances must be inventoried as they arrived to your lab The chemical inventory must be updated and every 6 months submitted to EHS Chemical substances must be arranged according to their physical/chemical characteristics Never arrange chemicals in alphabetical order in the shelves/cabinets. Its is a really bad practice 6/27/201118

19. 6/27/201119 Hazardous waste: Chemical Chemical waste includes:Chemical waste includes: –Materials that posses hazardous characteristics (e.g. toxic, flammable, corrosive, reactive, combustible) –Old chemicals no longer used –Chemicals past their expiration date –Chemicals with illegible labels or without labels, or cracked containers –Any chemical by-product from completed experiments

20. 6/27/201120 Inherently Waste Like Chemicals *RCRA strictly regulates chemical use, which includes considering obsolete chemicals to be Inherently Waste-Like. A chemical may be considered Inherently Waste Like if: –There is an over-abundance of a chemical in the laboratory. –The chemical container is damaged –The chemical container or label appear outdated –A label is damaged, discolored, or unreadable. –A chemical has not been used for 3 years *RCRA=resource conservation recovery act Pictured above are chemicals qualifying as Inherently Waste Like

21. 6/27/201121 Small Chemical Spill (100ml or less) Accidental release / CHEMICAL SPILLAccidental release / CHEMICAL SPILL –Call Security @ 594-4226 –Ventilate area of leak or spill –Remove sources of ignition –Wear PPE –Isolate hazard area –Locate Chemical Spill Kit –Contain and recover liquid when possible –Absorb with inert material and place in a chemical waste container –Label the waste container (name of chemical and date) –Place in Satellite Accumulation Area (SAA)–grey bin –

22. 6/27/201122 Chemical waste labeling/disposal LABELING: containers that store hazardous wasteLABELING: containers that store hazardous waste MUST BE properly and clearly labeled: Label should include: The words;HAZARDOUS WASTEThe words;HAZARDOUS WASTE Chemical names of all ingredientsChemical names of all ingredients –Hazard associated: Toxic, Corrosive DISPOSAL: all chemical waste containers should be put in the WASTE BIN or SAA (satellite accumulation area) for pick up byTriumvirateDISPOSAL: all chemical waste containers should be put in the WASTE BIN or SAA (satellite accumulation area) for pick up byTriumvirate Containers must be closed at all times when not adding waste. Do not mix incompatible wastes.

23. 6/27/201123 Hazardous waste: Radioactive Radioactive waste includes:Radioactive waste includes: –Radioisotopes stock vials –Any chemical by-product from completed experiments used with radioactive materials (e.g. buffers, polyacrylamide/agarose gels ) –Pipette tips, paper towel, transfer membranes –Pickup of radioactive waste is done as often as needed and left for decay and final disposal in the Radioactive Materials Waste Room

24. 6/27/201124 Hazardous waste: Pharmaceuticals The Environmental Protection Agency–EPA-The Environmental Protection Agency–EPA- –Protect public health and the environment by removing pharmaceuticals from waste water treatment plants and municipal solid waste landfills –Leave Pharmaceuticals in waste bins in labs or Satellite Accumulation Area –SAA-.

25. 6/27/201125 Recyclable waste-1 SINGLE STREAM RECYCLINGSINGLE STREAM RECYCLING Thanks to new technology, recyclable waste can be mixed at NYMC and put in BLUE BINS containers Acceptable items are:Acceptable items are: cardboard, magazines, office paper, junk mail, brown paper bags, paperboard, newspapers, plastic and glass bottles, aluminum cans and foil, tin cans, empty aerosol cans.

26. 6/27/201126 Recyclable waste-2 Fluorescent tubes and compact fluorescents lamps: because they may contain Mercury (Hg)Fluorescent tubes and compact fluorescents lamps: because they may contain Mercury (Hg) call EHS for info, x-4078call EHS for info, x-4078 Batteries must be put in special containers through out the NYMC halls/lobby

27. 6/27/201127 Recyclable waste-3 toner MUST BE sent back to manufacturer using free shipping labels toner MUST BE sent back to manufacturer using free shipping labels provided inside the box

28. 6/27/201128 E-Waste @ NYMC For disposal of Electronic Waste:For disposal of Electronic Waste: –Monitors -Printers -Faxes machines –Computers -Keyboards -Mouse Call the Office of Information-Services (IS) at X-4582 to arrange a pickupCall the Office of Information-Services (IS) at X-4582 to arrange a pickup

29. 6/27/201129 NON-Recyclable waste @ NYMC Food containers that are not cleanFood containers that are not clean Food wasteFood waste Styrofoam cupsStyrofoam cups Styrofoam boxes (some boxes can be returned to manufacturer with free shipping)Styrofoam boxes (some boxes can be returned to manufacturer with free shipping) Plastic bags (can be recyclable at local supermarket)Plastic bags (can be recyclable at local supermarket) WoodWood

30. 6/27/201130 Compressed Gas Cylinders-1 All compressed gas cylinders must be properly stored at all timesAll compressed gas cylinders must be properly stored at all times 1 1 2 2 1 2 3 BOTH STRAPS ARE SECURED BOTH STRAPS ARE SECURED ALL THREE STRAPS ARE SECURED

31. 6/27/201131 Compressed Gas Cylinders-2 All compressed gas cylinders should be stored with capAll compressed gas cylinders should be stored with cap Only one cylinder per bracket is allowedOnly one cylinder per bracket is allowed Cylinder cannot be stored with a gaugeCylinder cannot be stored with a gauge Cylinder should have a tag with name of investigator, room #, date, indicate if is FULL/ EMPTY/ IN USECylinder should have a tag with name of investigator, room #, date, indicate if is FULL/ EMPTY/ IN USE 12 NO WALL BRACKET TWO CYLINDERS ON ONE WALL BRACKET ONE OF THE STRAPS IS NOT SECURED

32. 6/27/201132 Compressed Gas Cylinders-3 Violation of store code Identification

33. 6/27/201133 Recombinant DNA (rDNA) Before engaging in any research or academic activity, experiments involving rDNA, (even exempt experiments) must be registered, reviewed by the College Biosafety Officer (BSO), and may have to be submitted for approval by the Institutional Biosafety Committee (IBC)Before engaging in any research or academic activity, experiments involving rDNA, (even exempt experiments) must be registered, reviewed by the College Biosafety Officer (BSO), and may have to be submitted for approval by the Institutional Biosafety Committee (IBC) Call EHS for info, x-4078Call EHS for info, x-4078

34. 6/27/201134 Allergies: Facts –Allergies have been found to be associated with most animals used in research labs –People develop allergic reaction to animal protein found in urine, dander, and saliva –70% of people with pre-existing allergies will develop a new allergy –If you have allergies let your supervisor know about it before your start working with animals Comparative Medicine Dept.

35. 6/27/201135 Autoclave Guidelines Training is a must for any person operating or handling an autoclave, it sterilizes by using High-Pressure Steam and High Temperature Conditions A practical training on Autoclave Safety from an experienced and knowledgeable person must be taken before using an autoclave Misuse can be very hazardous thus endangering human lives Always wear your personal protective –PPE- equipment when you are operating an autoclave

36. 6/27/201136 Biosafety Cabinets (BSC) Guidelines When working in the BSC you must:When working in the BSC you must: –Check the UV light is off –Make sure the sash is at the correct sash height –Avoid blocking the front and rear grills as they will decrease your personal protection as well as cleanliness of your experiment

37. 6/27/201137 Chemical Fume Hoods Guidelines Before starting work in the hood, check that it is functional, that there is sufficient air flow, test with small piece of kimwipes ® or small amount of dry ice mixed with water Check for certification, that its current Lower the sash to the proper operating position Work at least 4-6 inside hood Airflow in a fume hood is easily disrupted; do not use the fume hood for storage…!!! Hood not working? Report to Facilities @ 594-4588

38. 6/27/201138 Cold Rooms Guidelines Rules are for employee safety, to control MOLD and prevent contaminationRules are for employee safety, to control MOLD and prevent contamination No foodNo food No beveragesNo beverages To prevent Mold growth, keep all benches cleanTo prevent Mold growth, keep all benches clean No storage in No storage in Cardboard BoxesCardboard Boxes Paper ProductsPaper Products cell media

39. 6/27/201139 Darkrooms Guidelines Darkrooms Guidelines One of the most important rules in the darkroom is to keep it CLEAN and FREE of any paper waste and gels because the drain gets clogged thus provoking floods that will affects other adjacent areas.One of the most important rules in the darkroom is to keep it CLEAN and FREE of any paper waste and gels because the drain gets clogged thus provoking floods that will affects other adjacent areas. Any waste generated by the user MUST be taken to the lab and disposed of appropriatelyAny waste generated by the user MUST be taken to the lab and disposed of appropriately

40. 6/27/201140 Summary In order to work safely in the lab you must follow proper safety proceduresIn order to work safely in the lab you must follow proper safety procedures Basic rules of hygiene govern a friendly safe and healthy environmentBasic rules of hygiene govern a friendly safe and healthy environment Wastes generated in labs should be carefully monitored for disposalWastes generated in labs should be carefully monitored for disposal Recycling is one of your responsibilities as an employee of New York Medical CollegeRecycling is one of your responsibilities as an employee of New York Medical College

41. Done! Congratulations and now take a test by clicking on the link and follow the instructions: http://www.proprofs.com/quiz- school/story.php?title=laboratory-safety_7 http://www.proprofs.com/quiz- school/story.php?title=laboratory-safety_7 6/27/201141