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Identification of novel parvovirus B19 variants

Published byMarybeth Marsh Modified over 6 years ago

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Presentation on theme: "Identification of novel parvovirus B19 variants"— Presentation transcript:

1 Identification of novel parvovirus B19 variants
Lori Rinckel, PhD Talecris Biotherapeutics Raleigh Test Laboratory Nucleic Acid Technology Development

2 Detection of novel B19 variants
Investigational assay developed in house for parvovirus B19 research studies Assay performance assessed by testing clinical samples in parallel with test of record Assessment identified several novel variants of parvovirus B19 CONFIDENTIAL

3 Parvovirus B19 Variant ‘E3’
E3 = Genotype 1 variant: A6 Variant Genotype 2 E3 D91.1 Variant Genotype 3 B19 WT Genotype 1 V9 Variant Genotype 3 CONFIDENTIAL

4 Parvovirus B19 Variant ‘D11’
D11 = Genotype 1 variant: D11 CONFIDENTIAL

5 Parvovirus B19 Variant ‘P1’
P1 = Genotype 3 variant: P1 V9 Variant Genotype 3 D91.1 Variant Genotype 3 A6 Variant Genotype 2 B19 WT Genotype 1 Series of donations from same donor also tested CONFIDENTIAL

6 ‘P1’ Relative Titer CONFIDENTIAL

7 Parvovirus B19 Phylogeny
D11 Variants Variants Variants differ in DNA sequence by < 10% Genotypes differ in DNA sequence by ~10% Others ??? Variants Where did Lori get the 10% value??? Provisional patent on D11…how much work do we want to do with these odd ones…what is the value in chasing these down? Lori, we just want to see how our assay works…Todd, may not be worth a patent…publications, etc. V9 Variants P1 D91.1 Candotti et al 2004 CONFIDENTIAL

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