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Submitochondrial localization of mitofilin using quantitative immunoelectron microscopy.
Submitochondrial localization of mitofilin using quantitative immunoelectron microscopy. (A) Immunogold labeling of mitofilin in HeLa cells. A representative mitochondrion is shown. The arrow points to the position of a gold particle. (B) Localizations of the gold particles (red) as determined by immunogold labeling of mitofilin plotted on a scheme representing a part of a mitochondrion. The histogram shows the fraction of gold particles within the indicated distance to the crista junction. The histogram and the graphical representation are based on the same measured gold particle localizations. OM, outer membrane; IM, inner membrane. (Scale bar: 100 nm.) Jans D C et al. PNAS 2013;110: ©2013 by National Academy of Sciences
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