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Volume 29, Issue 6, Pages (June 2014)

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1 Volume 29, Issue 6, Pages 632-634 (June 2014)
Emerging from the Clouds: Vasa Helicase Sheds Light on piRNA Amplification  Julie M. Claycomb  Developmental Cell  Volume 29, Issue 6, Pages (June 2014) DOI: /j.devcel Copyright © 2014 Elsevier Inc. Terms and Conditions

2 Figure 1 Model of the Amplifier Complex Function in piRNA Biogenesis
TE clusters are transcribed, and transcripts are exported with the aid of UAP56, then converted to primary antisense piRNAs by the nuclease Zucchini. Antisense piRNAs are loaded into Aub/Siwi complexes, which target sense TE transcripts (piRNA precursors). Vasa interacts with the sense piRNA precursor and Ago3, Qin/Kumo, and Siwi. Siwi slices the piRNA precursor, liberating a portion for degradation and creating a piRNA intermediate associated with Ago3. Ago3 and mature sense piRNAs (trimmed and methylated) then target antisense transcripts in a similar manner during the ping-pong cycle. Developmental Cell  , DOI: ( /j.devcel ) Copyright © 2014 Elsevier Inc. Terms and Conditions

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