1. Volume 122, Issue 3, Pages 784-788 (March 2002)
Molecular evidence for the same clonal origin of both components of an adenosquamous Barrett carcinoma 
Bastiaan P. van Rees, Remigio W. Rouse, Mireille J. de Wit, Carel J.M. van Noesel, Guido N.J. Tytgat, J.Jan B. van Lanschot, G.Johan A. Offerhaus 
Gastroenterology 
Volume 122, Issue 3, Pages (March 2002)
DOI: /gast
Copyright © 2002 American Gastroenterological Association Terms and Conditions

2. Fig. 1
(A) Macroscopy of the resected specimen: the tumor is indicated by an arrow. SM, preexistent squamous cell mucosa of the esophagus; BE, Barrett esophagus; GM, gastric mucosa. A small patch of squamous cell mucosa adjacent to the tumor is indicated by an asterisk. (B) Microscopy of the adenosquamous carcinoma; white arrows indicate the squamous cell carcinoma component, black arrows point to the adenocarcinoma component (H&E). (C) Microscopy of a representative part of the adenocarcinoma component that was microdissected and used for the p53 mutation analysis and microsatellite analyses (H&E). (D) Positive staining for the cytokeratin marker Cam 5.2 in the adenocarcinoma component. (E) Microscopy of a representative part of the squamous cell carcinoma component that was microdissected and used for the p53 mutation analysis and microsatellite analyses (H&E). (F) The squamous cell carcinoma component is negative for Cam 5.2.
Gastroenterology  , DOI: ( /gast )
Copyright © 2002 American Gastroenterological Association Terms and Conditions

3. Fig. 2
(A) Representative examples of the microsatellite marker analysis. The results of markers at loci on chromosomal arms 9p (D9S259), 16q (D16S2624), and 3p (D3S1478) are shown for normal lymphocytes (N), Barrett epithelium (BE), adenocarcinoma (AC), and squamous cell carcinoma (SC) component. The alleles are highlighted by arrows and the AIF is indicated in red, where applicable. There is LOH of the larger allele at marker D9S259 in BE, AC, and SC. At marker D16S2624 there is a shift only in the AC component (red arrowhead). There is LOH of the larger allele at marker D3S1478 both in the AC and in the SC component. (B and C) Positive staining for p53 in both the (B) AC component and (C) the SC component. (D) Sequencing of the p53 gene revealed the same T→A missense mutation in codon 163 in both the AC and the SC component (black arrowheads).
Gastroenterology  , DOI: ( /gast )
Copyright © 2002 American Gastroenterological Association Terms and Conditions

4. Fig. 3
Schematic representation of the postulated tumor progression pathway in this case of adenosquamous carcinoma. LOH at chromosomal arm 9p has occurred as an early event because it was found already in Barrett epithelium and is shared by both the SC and the AC component. LOH at chromosomal arms 3p, 5q, 10q, 14q, and 18q and the p53 point mutation occurred before the divergence because these events are observed in both components. A shift at marker D16S2624 is found only in the AC-component, and therefore must have occurred as a late event, after the divergence.
Gastroenterology  , DOI: ( /gast )
Copyright © 2002 American Gastroenterological Association Terms and Conditions