1. Cross-matching

2. Cross-matching
Cross-matching is performed to determine if the patient has antibodies that react with the donor's cells.
cross-matching can only be used if a patient has a negative antibody screen, which means that they do not have any active red blood cells typical antibodies.

11. 22% albumin: reduces zeta potential, bringing the RBCs closer together and enhancing agglutination.
The zeta potential (ζ-potential): indicates the degree of repulsion between adjacent, similarly charged particles ( between negatively charged RBCs)in a dispersion. For molecules and particles that are small enough, a high zeta potential will confer stability (no coagulation) i.e., the solution or dispersion will resist aggregation.
When the potential is low (like when using 22% Albumin enhancement media --- positive charge) , attraction exceeds repulsion and the dispersion will break (coagulation occur). So It increases antibody uptake and decreases incubation time

12. Anti-human globulin (IgG) enables red cells to cross-link so that agglutination is visible.
37˚C phase is required since IgG are warm acting antibodies.