1. Volume 130, Issue 4, Pages 1144-1152 (April 2006)
Predominance of Hepatitis B Virus YMDD Mutants Is Prognostic of Viral DNA Breakthrough 
Chang Hong Lee, Soo–Ok Kim, Kwan Soo Byun, Myung Soon Moon, Eun–Ok Kim, Jong Eun Yeon, Wangdon Yoo, Sun Pyo Hong 
Gastroenterology 
Volume 130, Issue 4, Pages (April 2006)
DOI: /j.gastro
Copyright © 2006 American Gastroenterological Association Institute Terms and Conditions

2. Figure 1
RFMP strategy. PCR were performed with primers containing a type IIS restriction endonuclease recognition sequence (GGATG; FokI) embedded 8 bases ahead of codon ATG of rtM204. The enzymatic digestion of the products released a pair of 7mer and 13mer fragments representing nucleotide sequences within the codons shown in bold italic, and then masses of the resulting oligonucleotide fragments were analyzed by the mass spectrometer. Cleavage sites of FokI and BstF5I, an isoschizomer for FokI, are indicated by solid and open triangles, respectively, and restriction endonuclease recognition sites and primers by shaded bar and shaded arrows, respectively. One-base gap (T at position 720) replaced by the artificial sequences is denoted by open space.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2006 American Gastroenterological Association Institute Terms and Conditions

3. Figure 2
The accuracy of measuring relative abundance in mixed genotypes by RFMP. Test was done for linearity between the mixing ratios and the corresponding 13mer peak ratios across artificial pools with different proportions of genotypes ranging from 5% to 95%. Expected percentages denote [rt204I/(rt204I+rt204M)] × 100. The error bars represent the standard deviation. A diagonal line would be expected for complete concordance between measured and real values. R2 = Slope = The slope was not significantly different from 1 (P = .810).
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2006 American Gastroenterological Association Institute Terms and Conditions

4. Figure 3
Distribution of 429 HBV DNA-negative sera by relative abundance of YMDD mutant over wild-type viruses determined by RFMP analysis. Four hundred twenty-nine serum samples were collected longitudinally from 116 patients during a median 21 months of lamivudine therapy. The 429 samples were found to be HBV DNA negative when tested by the Digene Hybrid Capture assay (<1.4 × 105 copies/mL) and were tested for YMDD genotype by RFMP and classified according to relative abundance (fold excess) of mutant against wild-type viruses into 7 groups in ascending order; 165 sera of 0 (no mutant detected), 120 sera of less than 1, 103 sera of less than 2.5, 25 sera of less than 5, 10 sera of less than 7.5, 3 sera of less than 10, and 3 sera of 10 or over (including mutant only). Number in parentheses and solid part of a bar denote the cases of showing HBV DNA breakthrough within 6 months of genotyping.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2006 American Gastroenterological Association Institute Terms and Conditions

5. Figure 4
Definition of predominant genotypes in various mixed infections with wild-type and mutant viruses. Upper spectra indicate mixed genotypes of wild-type and mutant viruses, one of which does not dominate to reach the citeria for definition of predominance, at least 5-fold higher amount of one genotype than the other, and lower spectra predominant Ile (left) or predomiant Val (right). AI and m/z represent absolute peak intensity (×108 counts) and mass to charge ratio, respectively. Actual peak counts of 13mers were shown in parentheses.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2006 American Gastroenterological Association Institute Terms and Conditions