1. Ex vivo evaluation of cytotoxicity and melanocyte viability after A-101 hydrogen peroxide topical solution 40% or cryosurgery treatment in seborrheic keratosis lesions 
Stephanie Kao, BA, Alexi Kiss, MD, Tatiana Efimova, PhD, Adam Friedman, MD 
Journal of the American Academy of Dermatology 
Volume 79, Issue 4, Pages (October 2018)
DOI: /j.jaad
Copyright © 2018 American Academy of Dermatology, Inc. Terms and Conditions

2. Fig 1
Cell viability was assessed via TUNEL (terminal deoxynucleotidyl transferase dUTP nick-end labeling) immunohistochemical staining (A) and MTT [3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide] assay (B). MRHE tissues of a 9-mm diameter were fixed in 10% formaldehyde and paraffin embedded. Cell counts to quantify TUNEL-positive cells were performed at high power (40×) from 20 representative fields using a randomly selected 5 samples per group. MTT assay data is representative of 4 independent experiments from 4 different MRHE tissues. C, S100 immunohistochemical stainings of MRHEs treated with cryosurgery or A-101 were used to assess the number of melanocytes. Cell counts were performed at high power (40×) with 20 representative fields using a randomly selected 5 samples per group. HPF, High-power field; ns, not significant. *P < .05; **P < .01.
Journal of the American Academy of Dermatology  , DOI: ( /j.jaad )
Copyright © 2018 American Academy of Dermatology, Inc. Terms and Conditions