1. The role of integrins in saphenous vein vascular smooth muscle cell migration 
Hiroyuki Itoh, MD, Peter R. Nelson, MD, Leila Mureebe, MD, Arie Horowitz, DSc, K.Craig Kent, MD 
Journal of Vascular Surgery 
Volume 25, Issue 6, Pages (June 1997)
DOI: /S (97)
Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

2. Fig. 1
Immunofluorescence staining of integrins and subunits. SMCs were seeded onto plastic slides at a density of 5000 cells/cm2 in media that contained 10% FBS and were allowed to attach overnight. After incubation in serum-free media for 24 hours, cells were fixed with 2% paraformaldehyde and then incubated for 24 hours with primary antibody to β1 (A), α2 (B), α5 (C), and αvβ3 (D). Slides were then exposed to biotinylated goat antimouse immunoglobulin G, followed by Streptavidin-Texas Red. Cells, after fixation, were permeablized with 0.1% Triton X-100 for 10 minutes before staining with the αvβ3 integrin.
Journal of Vascular Surgery  , DOI: ( /S (97) )
Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

3. Fig. 1
Immunofluorescence staining of integrins and subunits. SMCs were seeded onto plastic slides at a density of 5000 cells/cm2 in media that contained 10% FBS and were allowed to attach overnight. After incubation in serum-free media for 24 hours, cells were fixed with 2% paraformaldehyde and then incubated for 24 hours with primary antibody to β1 (A), α2 (B), α5 (C), and αvβ3 (D). Slides were then exposed to biotinylated goat antimouse immunoglobulin G, followed by Streptavidin-Texas Red. Cells, after fixation, were permeablized with 0.1% Triton X-100 for 10 minutes before staining with the αvβ3 integrin.
Journal of Vascular Surgery  , DOI: ( /S (97) )
Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

4. Fig. 1
Immunofluorescence staining of integrins and subunits. SMCs were seeded onto plastic slides at a density of 5000 cells/cm2 in media that contained 10% FBS and were allowed to attach overnight. After incubation in serum-free media for 24 hours, cells were fixed with 2% paraformaldehyde and then incubated for 24 hours with primary antibody to β1 (A), α2 (B), α5 (C), and αvβ3 (D). Slides were then exposed to biotinylated goat antimouse immunoglobulin G, followed by Streptavidin-Texas Red. Cells, after fixation, were permeablized with 0.1% Triton X-100 for 10 minutes before staining with the αvβ3 integrin.
Journal of Vascular Surgery  , DOI: ( /S (97) )
Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

5. Fig. 1
Immunofluorescence staining of integrins and subunits. SMCs were seeded onto plastic slides at a density of 5000 cells/cm2 in media that contained 10% FBS and were allowed to attach overnight. After incubation in serum-free media for 24 hours, cells were fixed with 2% paraformaldehyde and then incubated for 24 hours with primary antibody to β1 (A), α2 (B), α5 (C), and αvβ3 (D). Slides were then exposed to biotinylated goat antimouse immunoglobulin G, followed by Streptavidin-Texas Red. Cells, after fixation, were permeablized with 0.1% Triton X-100 for 10 minutes before staining with the αvβ3 integrin.
Journal of Vascular Surgery  , DOI: ( /S (97) )
Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

6. Fig. 2
Effect of β1 integrin antibody on SMC migration induced by ECM proteins and PDGF-BB. Chemotaxis of human SMCs incubated with collagen I (CNI; 1 μg/ml), collagen IV (CNIV; 1 μg/ml), laminin (LN; 20 μg/ml), fibronectin (FN; 20 μg/ml), vitronectin (VN; 20 μg/ml), and PDGF-BB (5 ng/ml), with (hatched bar) and without (filled bar) antibody to the β1 subunit (dilution 1:1500). Results are expressed as a percentage of stimulated control ± SD. Experiments were performed in triplicate and repeated in multiple cell lines. Data from a representative experiment are shown. *p < 0.05 compared with control specimen.
Journal of Vascular Surgery  , DOI: ( /S (97) )
Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

7. Fig. 3
Effects of α2 integrin antibody on SMC migration induced by ECM proteins and PDGF-BB. Chemotaxis of human SMCs incubated with collagen I (CNI; 1 μg/ml), collagen IV (CNIV; 1 μg/ml), laminin (LN; 20 μg/ml), fibronectin (FN; 20 μg/ml), vitronectin (VN; 20 μg/ml) and PDGF-BB (5 ng/ml), with (hatched bar) and without (filled bar) antibody to the α2 subunit (dilution 1:6400). Results are expressed as a percentage of stimulated control ± SD. Experiments were performed in triplicate and repeated in multiple cell lines. Data from a representative experiment are shown. *p < 0.05 compared with control specimen.
Journal of Vascular Surgery  , DOI: ( /S (97) )
Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

8. Fig. 4
Effects of α5 integrin antibody on SMC migration induced by ECM proteins and PDGF-BB. Chemotaxis of human SMCs incubated with collagen I (CNI; 1 μg/ml), collagen IV (CNIV; 1 μg/ml), laminin (LN; 20 μg/ml), fibronectin (FN; 20 μg/ml), vitronectin (VN; 20 μg/ml) and PDGF-BB (5 ng/ml), with (hatched bar) and without (filled bar) antibody to the α5 subunit (dilution 1:1500). Results are expressed as a percentage of stimulated control ± SD. Experiments were performed in triplicate and repeated in multiple cell lines. Data from a representative experiment are shown.
Journal of Vascular Surgery  , DOI: ( /S (97) )
Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

9. Fig. 5
Effects of αvβ3 integrin antibody on SMC migration induced by ECM proteins and PDGF-BB. Chemotaxis of human SMCs incubated with collagen I (CNI; 1 μg/ml), collagen IV (CNIV; 1 μg/ml), laminin (LN; 20 μg/ml), fibronectin (FN; 20 μg/ml), vitronectin (VN; 20 μg/ml) and PDGF-BB (5 ng/ml), with (hatched bar) and without (filled bar) antibody to the αvβ3 integrin (dilution, 1:100). Results are expressed as a percentage of stimulated control ± SD. Experiments were performed in triplicate and repeated in multiple cell lines. Data from a representative experiment are shown. *p < 0.05 compared with control specimen.
Journal of Vascular Surgery  , DOI: ( /S (97) )
Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions