1. Volume 137, Issue 2, Pages 598-606.e2 (August 2009)
Release of Endogenous Opioids From Duodenal Enteroendocrine Cells Requires Trpm5 
Zaza Kokrashvili, Deniliz Rodriguez, Valeriya Yevshayeva, Hang Zhou, Robert F. Margolskee, Bedrich Mosinger 
Gastroenterology 
Volume 137, Issue 2, Pages e2 (August 2009)
DOI: /j.gastro
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2. Figure 1
(A–C) Sections from duodena of Trpm5-GFP mice. (A) GFP fluorescence, (B) indirect immunostaining with Trpm5 antibody, and (C) overlay. (D) GFP fluorescence in freshly dissected “live” epithelium of jejunum. Higher-magnification view shows the typical shape of Trpm5-GFP cells in sections of (E) duodenum and (F) colon. Bars, 20 μM.
Gastroenterology  , e2DOI: ( /j.gastro )
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3. Figure 2
Sections of duodenum from Trpm5-GFP mice. (A and D) GFP fluorescence, immunostaining with (B) chromogranin A and (E) chromogranin B antibody, and (C and F) overlays. Bars, 20 μM.
Gastroenterology  , e2DOI: ( /j.gastro )
Copyright © 2009 AGA Institute Terms and Conditions

4. Figure 3
Sections of duodenum from Trpm5-GFP mice. (A and D) GFP fluorescence, immunostaining with antibodies against (B) Met-enkephalin, (E) β-endorphin, and (C and F) overlays. Insets show higher magnification of cells from a different field. Bars, 20 μM.
Gastroenterology  , e2DOI: ( /j.gastro )
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5. Figure 4
Sections of duodenum from Trpm5-GFP mice. (A and D) GFP fluorescence, (B and E) immunostaining with antibodies against uroguanylin, and (C and F) overlays. Insets show higher magnification of cells. Bars, 20 μM
Gastroenterology  , e2DOI: ( /j.gastro )
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6. Figure 5
β-endorphin concentration determined by enzyme-linked immunosorbent assay in samples collected in 1-minute intervals from duodenum-jejunum perfused with 100 mmol/L NaCl in (A) HBSS, (B) 300 mmol/L glucose in HBSS, (C) 20 mmol/L sucralose in HBSS, (D) 20% Intralipid, and (E) 5% mannitol in HBSS. Control samples were collected after 3–5 minutes of perfusion with HBSS only (P). Four animals were tested in each group (see Materials and Methods). *P < .05, **P < .01, ***P < .001 compared with preincubation values (P).
Gastroenterology  , e2DOI: ( /j.gastro )
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7. Supplementary Figure 1
GFP fluorescence in freshly isolated villus from ileum compared with right-field image.
Gastroenterology  , e2DOI: ( /j.gastro )
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8. Supplementary Figure 2
GFP fluorescence in sections of Trpm5-GFP mice from (A) common bile duct and (B) papilla of Vater.
Gastroenterology  , e2DOI: ( /j.gastro )
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