1. ΔsimA yeast cells appear degraded in vivo.
ΔsimA yeast cells appear degraded in vivo. Transmission electron microscopy images of the wild-type (simA+) and ΔsimA strains 24 h postinfection of macrophages are shown. (A) After 24 h postinfection of macrophages, wild-type (simA+) yeast cells were observed within a macrophage phagosome. In contrast, the ungerminated ΔsimA conidia were not encompassed within an intracellular macrophage compartment. (B) In contrast to macrophages infected with the wild-type strain, macrophages infected with ΔsimA conidia contained numerous degraded cells. (C) The cell wall of wild-type (simA+) yeast cells appeared as at least two distinct defined layers: a thick dense layer lying directly adjacent to the lipid bilayer and a denser, unevenly distributed outer layer. Some ΔsimA conidia lacked clearly defined cell wall layers. Scale bars, 5 µm (A), 2 µm (B), and 0.5 µm (C).
Kylie J. Boyce et al. mSphere 2018; doi: /mSphere