1. Bone marrow-impregnated collagen matrix for wound healing: experimental evaluation in a microcirculatory model of angiogenesis, and clinical experience 
Shigeru Ichioka, Sachio Kouraba, Naomoi Sekiya, Norihiko Ohura, Takashi Nakatsuka 
British Journal of Plastic Surgery 
Volume 58, Issue 8, Pages (December 2005)
DOI: /j.bjps
Copyright © 2005 The British Association of Plastic Surgeons Terms and Conditions

2. Figure 1
Appearance of the new chamber. The chamber consists of two aluminum frames held together by four polyethylene tubes. One frame is equipped with a plastic sheath and three small screws, and the other is furnished with a glass window for observation. (a) Aluminum frame. (b) Hole drilled for polyethylene tube to hold the two frames together. (c) Glass window for intravital microscopic observation. (d) Plastic sheath. (e) Fixing screw. (f) Plastic cap.
British Journal of Plastic Surgery  , DOI: ( /j.bjps )
Copyright © 2005 The British Association of Plastic Surgeons Terms and Conditions

3. Figure 2
Skinfold chamber implantation. Transectional schema (above) and general appearance of a mouse with the chamber in place (below). The dorsal skin was drawn upward and a square area of one layer of skin has been removed. The frames were implanted so as to sandwich the stretched double layer of the skin between them. The remaining layer consists of skin (a) and a thin layer of striated skin muscle (b) covered with glass (c).
British Journal of Plastic Surgery  , DOI: ( /j.bjps )
Copyright © 2005 The British Association of Plastic Surgeons Terms and Conditions

4. Figure 3
Wound creation procedure. Transectional schema (above) and appearance under an operating microscope (below). A circular area of skin was excised with microforceps and scissors down to, but not including, the underlying striated skin muscle layer. A dressing (a) covered the wound, and the plastic cap (b) fixed the dressing.
British Journal of Plastic Surgery  , DOI: ( /j.bjps )
Copyright © 2005 The British Association of Plastic Surgeons Terms and Conditions

5. Figure 4
Intravital microscopic appearance of the whole window immediately after creation of the wound (left) and the magnified image of the wound with surrounding vessels designated as the region of interest (right). The wound (a) was located in the area supplied by the third order of arterioles (A3).
British Journal of Plastic Surgery  , DOI: ( /j.bjps )
Copyright © 2005 The British Association of Plastic Surgeons Terms and Conditions

6. Figure 5
Changes in microvasculature in the same animal. Photomicrographs (above) and the computer-processed binary images demarcating functional microvessels (below) on day 3 (A), day 5 (B), day 7 (C), and day 10 (D) after wounding.
British Journal of Plastic Surgery  , DOI: ( /j.bjps )
Copyright © 2005 The British Association of Plastic Surgeons Terms and Conditions

7. Figure 6
Percentage increase in FCD after wound creation in control and bone marrow applied animals. *Significantly different at p<0.05 vs controls. **Significantly different at p<0.005 vs controls.
British Journal of Plastic Surgery  , DOI: ( /j.bjps )
Copyright © 2005 The British Association of Plastic Surgeons Terms and Conditions

8. Figure 7
(A) Nonhealing ulcer on the left medial malleolus prior to treatment and before wound debridement. (B) Appearance immediately after debridement and prior to application of bone marrow suspension (C) Good granulation tissue 2 weeks after bone marrow application. (D) Healed wound following treatment of bone marrow followed by a split-thickness mesh skin graft.
British Journal of Plastic Surgery  , DOI: ( /j.bjps )
Copyright © 2005 The British Association of Plastic Surgeons Terms and Conditions