1. Cumulus cell transcriptome profiling is not predictive of live birth after in vitro fertilization: a paired analysis of euploid sibling blastocysts 
Katherine A. Green, M.D., Jason M. Franasiak, M.D., T.S., Marie D. Werner, M.D., H.C.L.D., Xin Tao, M.S., Jessica N. Landis, Ph.D., Richard T. Scott, M.D., H.C.L.D., Nathan R. Treff, Ph.D. 
Fertility and Sterility 
Volume 109, Issue 3, Pages e2 (March 2018)
DOI: /j.fertnstert
Copyright © Terms and Conditions

2. Figure 1
Heatmap of sample-to-sample Euclidean distances using the regularized-logarithm (rlog) transformation of gene counts, with the dendrogram showing hierarchical sample clustering. LB = live birth; N = no live birth.
Fertility and Sterility  , e2DOI: ( /j.fertnstert )
Copyright © Terms and Conditions

3. Figure 2
Normalized gene count of VCAN [plotted as log2(normalized count)] from cumulus cells for each embryo pair. No significant differences (FDR <.05) in VCAN expression in cumulus cells were detected between sibling embryos resulting in live birth vs. no live birth.
Fertility and Sterility  , e2DOI: ( /j.fertnstert )
Copyright © Terms and Conditions

4. Supplemental Figure 1
Principle component analysis plot of gene expression data from cumulus cells of sibling embryos resulting in live birth vs. no live birth using regularized-logarithm transformation (rlog) values as implemented in DESeq2.
Fertility and Sterility  , e2DOI: ( /j.fertnstert )
Copyright © Terms and Conditions

5. Supplemental Figure 2
Power of increasing biological replicates in RNASeq. Increasing biological replicates significantly increases the number of differentially expressed (DE) genes. There are diminishing returns when sequencing to depths >10 × 106 reads. Figure reprinted with permission from Liu et al. (11).
Fertility and Sterility  , e2DOI: ( /j.fertnstert )
Copyright © Terms and Conditions