1. Mock ICSBP
Snail
Slug
Twist
β-actin
Suppl. Fig. 1

2. Suppl. Fig. 2 A si-ICSBP+IFN-g si-Cont+IFN-g si- Cont ICSBP TGF-β RI
ICSBP/actin
TGF-β RI
TGF-β RI/actin
b-actin B
Cont IFN-g
Snail
TGF-β RI
b-actin
Suppl. Fig. 2

3. A
Cell number ( X10 4 ) B
Total TGF-β (ng/mL)
Suppl. Fig. 3

5. Suppl. Fig. 5 Nasopharyngeal carcinoma tissue Osteosarcoma tissueB
B -lymphocyte
Macrophage
Osteosarcoma tissue
Suppl. Fig. 5

6. Suppl. Fig. 6 Suppl. Fig. 6 SiHa SiHa-Mock SiHa-ICSBP SiHa-Mock
Mock ICSBP
ICSBP
Snail
SiHa
β-actin A
SiHa-Mock
SiHa-ICSBP B
E-cadherin
100μm
100μm C
SiHa-Mock
SiHa-ICSBP
Suppl. Fig. 6
Suppl. Fig. 6

7. Suppl. Fig. 7 Snail A B Negative Snail score Weak r = 0.2319
ICSBP score
Intermediate C
Snail score
Strong
r =
p =
TGF-b RI score
Positive control
(kidney)
Negative control (fibroblast tissue)
Suppl. Fig. 7

8. Suppl. Fig. 8 ICSBP Activation of TGF-b signaling
Up-regulation of Snail
Down regulation of ZO-1 and E-cadherin, and up-regulation of fibronectin and vimentin
ELP and increased cell motility and invasiveness
Up-regulation of Slug and Twist
Suppl. Fig. 8