Download presentation
Presentation is loading. Please wait.
1
Differential expression and regulation of prostaglandin transporter and metabolic enzymes in mouse uterus during blastocyst implantation Fei Gao, M.S., Wei Lei, B.S., Hong-Lu Diao, Ph.D., Shi-Jun Hu, B.S., Li-Ming Luan, Ph.D., Zeng-Ming Yang, Ph.D. Fertility and Sterility Volume 88, Issue 4, Pages (October 2007) DOI: /j.fertnstert Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions
2
Figure 1 In situ hybridization of PGT mRNA in mouse uteri during pregnancy on days 1 (A), 4 (B), 5 (C, implantation [IM] site), 5 (D, interimplantation [NIM] site), 6 (F), 7 (G), and 8 (H). There was no detectable PGT mRNA in mouse uterus on day 5 of pseudopregnancy (E). Prostaglandin transporter mRNA was strongly detected in the subluminal stroma surrounding the implanting blastocyst on day 5 (C) and decidua during days 6 to 8 of pregnancy (F–H). Arrows, embryos. Bars = 60 μm. Gao. PGT in mouse embryo implantation. Fertil Steril 2007. Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions
3
Figure 2 Prostaglandin transporter immunofluorescence in mouse uteri during pregnancy on days 1 (A), 4 (B), 5 (C), 6 (D), 7 (E), and 8 (F). Prostaglandin transporter signal was not detected at the site of blastocyst apposition on day 4 (B) but was strongly seen in the subluminal stroma surrounding the implanting embryo on day 5 (C) and in decidua from days 6 to 8 (D–F). ∗Embryos. Bars = 60 μm. Gao. PGT in mouse embryo implantation. Fertil Steril 2007. Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions
4
Figure 3 There was no detectable PGT mRNA (A) and protein (C) at the site of blastocyst apposition in mouse uteri during delayed implantation. After delayed implantation was terminated by estrogen treatment and embryo implanted, both PGT mRNA (B) and protein (D) were strongly observed in the subluminal stroma surrounding the implanting blastocyst. ∗Embryos. Bars = 60 μm. Gao. PGT in mouse embryo implantation. Fertil Steril 2007. Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions
5
Figure 4 In situ hybridization of 15-PGDH mRNA in mouse uteri during pregnancy on days 4 (A), 5 (B, implantation [IM] site), 5 (C, interimplantation [NIM] site), 7 (E), and 8 (F). There was no detectable 15-PGDH mRNA in mouse uterus on day 5 of pseudopregnancy (D). Specific 15-PGDH mRNA signals were detected in the luminal epithelium surrounding the implanting blastocyst on day 5 (C) and embryos on days 7 and 8 of pregnancy (E, F). ∗Embryos. Bars = 60 μm. Gao. PGT in mouse embryo implantation. Fertil Steril 2007. Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions
6
Figure 5 In situ hybridization of CBR1 mRNA in mouse uteri during pregnancy on days 1 (A), 3 (B), 4 (C), 5 (D), 7 (E), and 8 (F). Only a basal level of CBR1 expression was seen in the luminal epithelium on day 5 (D). Specific PGT mRNA signals were detected in embryos on days 7 and 8 of pregnancy (E, F). ∗Embryos. Bars = 60 μm. Gao. PGT in mouse embryo implantation. Fertil Steril 2007. Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions
Similar presentations
© 2025 SlidePlayer.com. Inc.
All rights reserved.