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Supplementary table S1 by Shin et al.

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1 Supplementary table S1 by Shin et al.
Supplementary table S1. Primer sequences used for overexpression and deletion mutant of hnRNPK Vectors Forward primer Reverse primer FLAG-hnRNPK-FL GAATTCATGGAAACTGAACAGCCA CCCTCGAGGGTTAGAATCCTTCAAC FLAG-hnRNPK-DKH1 GAATTCGAAGAGGGCCTGCAGTTG FLAG-hnRNPK-DKH1/2 GAATTCGGAAGAGGTGGTTTTGA FLAG-hnRNPK-DKH2 CACTATAAAGGAAGTGACTTTGAGTCTCCC ACGTCCTTTGATGGGAGACTCAAAGTCACT FLAG-hnRNPK-DKH3 CCCTCGAGGGGTAACGGTCTCC

2 Supplementary table S2 by Shin et al.
Supplementary table S2. siRNA sequences used for knockdown of hnRNPK and PLK1 siRNA Sense Antisense hnRNPK siRNA (#1: CDS) GCAUAAAGAUCAUCCUUGA(TT) UCAAGGAUGAUCUUUAUGC(TT) hnRNPK siRNAS (#2: 3'UTR) CCAACAUUCCUCUGCUUCA(TT) UGAAGCAGAGGAAUGUUGG(TT) PLK1 siRNA (#1) AUGAAGAUCUGGAGGUGAA(TT) UUCACCUCCAGAUCUUCAU(TT) PLK1 siRNA (#2) GGAUCAAGAAGAAUGAAUA(TT) UAUUCAUUCUUCUUGAUCC(TT)

3 Supplementary table S3 by Shin et al.
Supplementary table S3. Antibodies used for western blot analyses and immunoprecipitation Antibody Company Cat. No MW hnRNP K(D-6) Santa Cruz sc-28380 65 kD hnRNP K(ChIP Grade) abcam Ab70492 PLK1(F-8) sc-17783 67 kD GFP(B34) sc-73556 27 kD FLAG(M2) Sigma F3165 Ago2 SAB 85 kD Cleaved PARP(Asp214) Cell Signaling Technology #9541 89 kD GAPDH(6C5) ab8245 37 kD

4 Supplementary table S4 by Shin et al.
Supplementary table S4. Primers used for RT-qPCR analyses Gene Forward Primer Reverse Primer hnRNP K GGCAGTGATTGGAAAAGGAG CACTGCTGTCTGGGACTGAA PLK1 GCCCCTCACAGTCCTCAATA GTCGACCACCTCACCTGTCT EGFP CGACAACCACTACCTGAGCA GAACTCCAGCAGGACCATGT GAPDH TGCACCACCAACTGCTTAGC GGCATGGACTGTGGTCATGAG 18S AACCCGTTGAACCCCATT CCATCCAATCGGTAGTAGCG

5 Supplementary table S5 by Shin et al.
Supplementary table S5. Primer sequences used for biotin pull-down assay Fragment Primers PLK1 3'UTR Fragment #1 Forward CCAAGCTTCTAATACGACTCACTATAGGGAGATAGCTGCCCTCCCCTC Reverse GCGGGAGCCAACCAGTATGGGCCCCAGATGCAG PLK1 3'UTR Fragment #2 CCAAGCTTCTAATACGACTCACTATAGGGAGAGGTGCCATGTCTGCAG GCAACCCCCACCTGCAAGGATGATGCAGCTCTG PLK1 3'UTR Fragment #3 CCAAGCTTCTAATACGACTCACTATAGGGAGATGTATAAGTTATTTTT ATTCATATGGTGGGGTTGAGGGGGAGGGGGACA PLK1 3'UTR Fragment #4 CCAAGCTTCTAATACGACTCACTATAGGGAGATGTACAGAATATTTCT GAATATTCACATCTGTTTAATGTGCATAAAGCC

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