1. Volume 130, Issue 7, Pages 2031-2038 (June 2006)
Prevalence of the Activating JAK2 Tyrosine Kinase Mutation V617F in the Budd–Chiari Syndrome 
Raj K. Patel, Nicholas C. Lea, Michael A. Heneghan, Nigel B. Westwood, Dragana Milojkovic, Murugaiyan Thanigaikumar, Deborah Yallop, Roopen Arya, Antonio Pagliuca, Joop Gäken, Julia Wendon, Nigel D. Heaton, Ghulam J. Mufti 
Gastroenterology 
Volume 130, Issue 7, Pages (June 2006)
DOI: /j.gastro
Copyright © 2006 American Gastroenterological Association Institute Terms and Conditions

2. Figure 1
Representative results of allele-specific PCR. The panel shows agarose gel electrophoresis of PCR products generated by allele-specific PCR performed on DNA from hematologically normal subjects and DNA extracted from patients with BCS. The presence of the lower 203-bp band indicates the presence of the V617F mutation. (Lanes 1–5) Wild-type controls all have a single band of 364 bp indicating a wild-type JAK2 allele. (Lanes 6, 7, and 10) BCS patient samples positive for the V617F mutation. (Lanes 8 and 9) BCS patient samples that are V617F negative. (Lane 11) V617-positive PV patient. (Lane 12) Hematologically normal subject. (Lane 13) No DNA control.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2006 American Gastroenterological Association Institute Terms and Conditions

3. Figure 2
Sequence traces of JAK2 mutant allele. Antisense sequences showing the G to T mutation (C to A in antisense) in JAK2. Traces are from PCR products generated from a hematologically normal control sample (wild type), a JAK2V617F mutant BCS patient sample (mixed) (sample from lane 6, Figure 1) and a PV patient carrying the V617F mutation (sample from lane 11 Figure 1). The arrow indicates the mutant allele peak underlying the wild-type sequence in the BCS sample.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2006 American Gastroenterological Association Institute Terms and Conditions