1. Molecular Cell Biology 5068
Bob Mercer, ;

2. Goals For MCB 5068
1. Obtain a solid foundation of knowledge in cell biology
2. Obtain a working knowledge of available techniques.
3. Be able to critically read and evaluate the scientific literature.
4. Be able to define and investigate a biological problem.

3. TA’s: Victoria Ismail rogersv@wustl.edu Aisha Lee aishalee@wustl.edu
Brittany Townley

4. Lectures: Tuesday and Thursday, 8:30-10:00 AM, Erlanger Auditorium
Molecular Cell Biology 5068
Lectures: Tuesday and Thursday, 8:30-10:00 AM, Erlanger Auditorium
9/18 & 10/11 in Holden Auditorium
Discussion Section: Wednesday, 3-4 PM
Papers assigned
One-page summary/critique expected

5. Molecular Cell Biology 5068
To Do:
Visit website:
Sign up for course
Check out Self Assessment homework
Visit Discussion Sections: Read “Official” Instructions

6. Molecular Cell Biology 5068

7. Discussion Section Techniques
Protein purification using GST Fusion
CRISPER/Cas 9 gene editing
SDS-PAGE/Immunoblotting/Immunoprecipitation
Immunofluorescence
Sanger Sequencing
Real time PCR
Proliferation Assay/BrdU
In vivo intracellular pH determination/pHluorin
Size exclusion chromatography

8. Grading: 3 in class closed book exams accounting for 75% of grade
25% of final grade based on performance in Discussion Section
Percentage final grades last 2 years:
36% A
28% A-
21% B+
14% B
0.5% B-
0.5% C+