1. Volume 4, Issue 4, Pages 283-290 (October 2006)
Release of small transmitters through kiss-and-run fusion pores in rat pancreatic β cells 
Patrick E. MacDonald, Matthias Braun, Juris Galvanovskis, Patrik Rorsman 
Cell Metabolism 
Volume 4, Issue 4, Pages (October 2006)
DOI: /j.cmet
Copyright © 2006 Elsevier Inc. Terms and Conditions

2. Figure 1
Cell-attached membrane capacitance measurements reveal the kiss-and-run exocytosis of single vesicles in rat β cells
A) Representative capacitance (Im/2πf) and conductance (Re) recordings from membrane patches of intact rat β cells stimulated with 10 mM glucose and 5 μM forskolin. Upward steps in Im/2πf result from the fusion of single vesicles within the patch. Some exocytotic steps were transient and followed by similar endocytotic (downward) Im/2πf steps (in parts ii and iv). Deflections in Re during exocytosis result from the presence of fusion pores. Adequate lock-in phase setting and separation of the Im/2πf and Re components is demonstrated by the deflection produced by pipette suction (end of part iv), which is apparent only in the Im/2πf trace.
B) The size distribution of exocytotic events, shown both as the square root of capacitance (fF1/2) and as vesicle diameter, demonstrating two vesicle populations corresponding to SLVs and LDCVs. Kiss-and-run events are shown in black.
C) The magnitudes of the upward (exocytotic) and downward (endocytotic) capacitance steps during transient vesicle fusions are highly correlated.
Cell Metabolism 2006 4, DOI: ( /j.cmet )
Copyright © 2006 Elsevier Inc. Terms and Conditions

3. Figure 2
Measurement of SLV fusion pore conductance during kiss-and-run exocytosis
A) A representative SLV kiss-and-run event. The true vesicle capacitance (Cv) and fusion pore conductance (Gp) were calculated from the Im and Re conductance components (see Experimental Procedures).
B) The distribution of fusion pore sizes, shown both as the square root of conductance (pS1/2) and as pore diameter. The dashed line represents the approximate diameter of GABA on the shortest axis.
Cell Metabolism 2006 4, DOI: ( /j.cmet )
Copyright © 2006 Elsevier Inc. Terms and Conditions

4. Figure 3
Measurement of LDCV fusion pore conductance and the effect of forskolin
A) A representative LDCV kiss-and-run event. The true vesicle capacitance (Cv) and fusion pore conductance (Gp) were calculated from the Im and Re conductance components (see Experimental Procedures).
B) The distribution of fusion pore sizes, shown both as the square root of conductance (pS1/2) and as pore diameter. The dashed lines represent the approximate diameter of ATP and insulin on the shortest axis.
C) A representative LDCV exocytotic event associated with a persistent conductance increase representing the presence of a fusion pore that neither closed nor expanded over the course of the experiment (up to 5 min). This was not due to an incorrect lock-in phase setting as demonstrated by the separation of Im/2πf and Re during pulses of pipette suction (end of Im/2πf trace). The true vesicle capacitance (Cv) and fusion pore conductance (Gp) were calculated from the Im and Re conductance components.
D) The relative occurrence of full LDCV fusion (i.e., nonreversible fusion with no persistent pore), LDCV kiss-and-run, and LDCV exocytosis with a persistent fusion pore.
E) The calculated diameters of transient LDCV fusion pores in the absence and presence of forskolin, and of persistent LDCV fusion pores. ∗∗−p < 0.01 compared with the kiss-and-run pores. All error bars represent the SEM.
Cell Metabolism 2006 4, DOI: ( /j.cmet )
Copyright © 2006 Elsevier Inc. Terms and Conditions

5. Figure 4
Quantal GABA release measurements reveal a subset of events conforming to release through a fusion pore
A) Representative current spikes from rat β cells expressing the GABAA receptor resulting from the quantal release of GABA from a single exocytotic SLV. Exocytosis was elicited by direct infusion of Ca2+ into the cell. The spike labeled i displays rapid kinetics while those labeled ii and iii display slowed kinetics. Scatter plots of current spike amplitude versus spike charge (B), and current spike half-width (t1/2) versus spike charge (C) are shown. Two distinct groups of quantal GABA release events are evident; one with rapid kinetics (closed circles) and a second with slowed kinetics (open circles).
Cell Metabolism 2006 4, DOI: ( /j.cmet )
Copyright © 2006 Elsevier Inc. Terms and Conditions

6. Figure 5
Quantal ATP release measurements reveal a subset of events conforming to release through a fusion pore
A) Representative current spikes from rat β cells expressing the P2X2 receptor resulting from the quantal release of ATP from a single exocytotic LDCV. Exocytosis was elicited by direct infusion of Ca2+ into the cell. The spike labeled i displays rapid kinetics while those labeled ii and iii display slowed kinetics. Scatter plots of current spike amplitude versus spike charge (B), and current spike half-width (t1/2) versus spike charge (C) are shown. Two distinct groups of quantal ATP release events are evident; one with rapid kinetics (closed circles) and a second with slowed kinetics (open circles). (D) In part i, a rapid expanding fusion pore is detected at the onset of LDCV exocytosis as a transient increase in Re. Rapid expansion of the pore is observed in the calculated fusion pore conductance (Gp). The simultaneous measurement of quantal ATP release and the amperometric measurement of 5-HT release reveal prespike feet during some release events that represent the exit of these molecules through the expanding fusion pore (part ii). Amperometric stand alone feet representing release of 5-HT through the kiss-and-run fusion pore was observed coincident with the slow kinetic It,ATP events (parts iii to iv).
Cell Metabolism 2006 4, DOI: ( /j.cmet )
Copyright © 2006 Elsevier Inc. Terms and Conditions

7. Figure 6
Kiss-and-run of LDCVs and SLVs are of sufficient duration to allow transmitter release
A) The total time to half-inactivation (t1/2 inact) of ATP and GABA current detection spikes, and the transient SLV and LDCV fusion times (tfusion) were calculated as indicated.
B) Average t1/2 inact for the fast and slow It,GABA spikes and tfusion for SLV kiss-and-run.
C) Cumulative histograms of the time distribution for rapid (closed circles) and slow (open circles) GABA events were compared to that for transient SLV fusions (open squares).
D) Average It,GABA spike charge for the fast and slow kinetic events.
E) The same as in (B), except for It,ATP spikes and LDCV kiss-and-run events.
F) Same as in (C), except comparing the time distribution of rapid (closed circles) and slow (open circles) ATP events to that for transient LDCV fusions (open squares).
G) Average It,ATP spike charge for the fast and slow kinetic events. ∗−p < 0.05 and ∗∗∗−p < compared with the first bar, unless indicated otherwise.
All error bars represent the SEM.
Cell Metabolism 2006 4, DOI: ( /j.cmet )
Copyright © 2006 Elsevier Inc. Terms and Conditions