1. Boulder Electron Microscopy Services
Serving University of Colorado labs, other universities and corporate researchers for 30+ years
Provides full range of transmission EM services
Conventional TEM, electron tomography and cryo-electron microscopy
Training: specimen preparation, microscope use, 3D software.
this will be illustrated by the published work displayed as the background of the slides I’ll show today
Kang et al., Traffic 12: , 2011

2. Boulder Electron Microscopy Services
Ueda et al., PNAS 108: , 2011.

3. Boulder Electron Microscopy Services: Conventional EM
Specimen preparation: HPF and freeze substitution
Thin section electron microscopy
Negative stain
Protein localization with immuno-gold
Erickson et al., PlosPathog 8:e , 2012

4. Boulder Electron Microscopy Services:
3D cellular tomography
Automated image acquisition using IVEMs
Software training for computing and modeling 3D reconstructions
O’Toole and Dutcher, Cytoskeleton, 71: , 2013

5. Boulder Electron Microscopy Services: Cryo-electron microscopy
Specimen preparation: Manual and Vitrobot plunge freezers.
Cryo-electron microscopy/tomography
3D volume averaging software
Cope et al., J Struct Biol. 170: , 2010

6. Boulder Electron Microscopy Services
Who we are and where we live:
Tom Giddings, Director
Mary Morphew
Courtney Ozzello
Eileen O’Toole
Department of MCD Biology, Porter Biosciences
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West et al., J. Cell Biol. 193:333-46, 2011.