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Contribution of IgE and immunoglobulin free light chain in the allergic reaction to cow's milk proteins  Bastiaan Schouten, PhD, Betty C.A.M. van Esch,

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Presentation on theme: "Contribution of IgE and immunoglobulin free light chain in the allergic reaction to cow's milk proteins  Bastiaan Schouten, PhD, Betty C.A.M. van Esch,"— Presentation transcript:

1 Contribution of IgE and immunoglobulin free light chain in the allergic reaction to cow's milk proteins  Bastiaan Schouten, PhD, Betty C.A.M. van Esch, BSc, Anders O.J. van Thuijl, MD, Bart R.J. Blokhuis, BSc, Tom Groot Kormelink, MSc, Gerard A. Hofman, BSc, Guido E. Moro, MD, PhD, Günther Boehm, MD, PhD, Sertac Arslanoglu, MD, PhD, Aline B. Sprikkelman, MD, PhD, Linette E.M. Willemsen, PhD, Léon M.J. Knippels, PhD, Frank A. Redegeld, PhD, Johan Garssen, PhD  Journal of Allergy and Clinical Immunology  Volume 125, Issue 6, Pages (June 2010) DOI: /j.jaci Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Specific serum immunoglobulin levels in casein- and whey-sensitized mice. In the casein-sensitized mice no increase in casein-specific IgE levels was found (A), but the IgG1 level was increased in 4 of 6 animals (B). Whey sensitization induced levels of specific IgE (C) and IgG1(D). ∗P < .05 and ∗∗P < .01 (n = 6). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Densitometric analysis of κ Ig-fLC in serum and supernatants from local mesenteric lymph nodes revealed Ig-fLC levels to be increased. A, Analysis of κ Ig-fLC in sera from individual mice. In casein-sensitized mice Ig-fLC levels were significantly increased in comparison with those seen in control- or whey-sensitized mice. B, Representative Western blot of Ig-fLC levels in supernatants of mesenteric lymph nodes of control-, casein-, and whey-sensitized mice. Ig-fLC, Monomers; Igs, total immunoglobulins (IgA, IgD, IgE, IgG, and IgM); (Lc)2, Ig-fLC dimers. ∗P < .05 (n = 6). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Effect of F991 on the induction of an acute allergen-specific skin reaction in casein-sensitized (A) and whey-sensitized (B) mice. F991 decreased casein-induced ear swelling significantly, but whey-induced ear swelling was unaffected. Ear swelling (in micrometers) is calculated as the increase in ear thickness induced by the corresponding antigen at 1 hour after challenge. ∗∗P < .01 and ∗∗∗P < .001 (n = 6). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Effect of F991 on the induction of an acute allergic skin reaction in naive mice injected intravenously with spleen supernatants or PBS as a control. Passive transfer with spleen supernatants of mice with casein (A) and whey (B) allergy resulted in an acute allergic skin reaction on intradermal allergen challenge. Only in casein recipients was the acute allergic skin reaction abrogated by F991. ∗∗∗P < .001 (n = 6). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 Plasma Ig-fLC levels in nonallergic control subjects (n = 17) and infants with CMA (n = 17). Both κ Ig-fLC levels (P = .0183; A) and λ Ig-fLC levels (P = .0328; B) were enhanced in infants with CMA. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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