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Biology
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2. 12–1 DNA EQ: What are the components of DNA?
Photo credit: Jacob Halaska/Index Stock Imagery, Inc.
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3. The Components and Structure of DNA
DNA is made up of nucleotides.
A nucleotide is a monomer of nucleic acids made up of:
Deoxyribose – 5-carbon Sugar
Phosphate Group
Nitrogenous Base
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4. The Components and Structure of DNA
There are four kinds of bases in in DNA:
adenine
guanine
cytosine
thymine
DNA is made up of nucleotides. Each nucleotide has three parts: a deoxyribose molecule, a phosphate group, and a nitrogenous base. There are four different bases in DNA: adenine, guanine, cytosine, and thymine.
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5. The Components and Structure of DNA
Chargaff's Rules
Erwin Chargaff discovered that:
The percentages of guanine [G] and cytosine [C] bases are almost equal in any sample of DNA.
The percentages of adenine [A] and thymine [T] bases are almost equal in any sample of DNA.
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6. The Components and Structure of DNA
X-Ray Evidence 
Rosalind Franklin used X-ray diffraction to get information about the structure of DNA.
She aimed an X-ray beam at concentrated DNA samples and recorded the scattering pattern of the X-rays on film.
This X-ray diffraction photograph of DNA was taken by Rosalind Franklin in the early 1950s. The X-shaped pattern in the center indicates that the structure of DNA is helical.
Photo credit: ©Cold Spring Harbor Laboratory Archives/Peter Arnold, Inc.
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7. The Components and Structure of DNA
The Double Helix 
Using clues from Franklin’s pattern, James Watson and Francis Crick built a model that explained how DNA carried information and could be copied.
Watson and Crick's model of DNA was a double helix, in which two strands were wound around each other.
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8. The Components and Structure of DNA
DNA Double Helix
DNA is a double helix in which two strands are wound around each other. Each strand is made up of a chain of nucleotides. The two strands are held together by hydrogen bonds between adenine and thymine and between guanine and cytosine.
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9. The Components and Structure of DNA
Watson and Crick discovered that hydrogen bonds can form only between certain base pairs—adenine and thymine, and guanine and cytosine.
This principle is called base pairing.
Adenine pairs with Thymine. A with T.
Cytosine pairs with Guanine. C with G.
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DNA and Chromosomes
Chromosome Structure
Eukaryotic chromosomes contain DNA and protein, tightly packed together to form chromatin.
Chromatin consists of DNA tightly coiled around proteins called histones.
DNA and histone molecules form nucleosomes.
Nucleosomes pack together, forming a thick fiber.
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DNA Replication
DNA Replication
Each strand of the DNA double helix has all the information needed to reconstruct the other half by the mechanism of base pairing.
In most prokaryotes, DNA replication begins at a single point and continues in two directions.
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DNA Replication
During DNA replication, the DNA molecule separates into two strands, then produces two new complementary strands following the rules of base pairing. Each strand of the double helix of DNA serves as a template for the new strand.
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DNA Replication
New Strand
Original strand
Nitrogen Bases
Growth
Growth
During DNA replication, the DNA molecule produces two new complementary strands. Each strand of the double helix of DNA serves as a template for the new strand.
Replication Fork
Replication Fork
DNA Polymerase
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DNA Replication
How Replication Occurs
DNA replication is carried out by enzymes that “unzip” a molecule of DNA.
Hydrogen bonds between base pairs are broken and the two strands of DNA unwind.
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DNA Replication
The principal enzyme involved in DNA replication is DNA polymerase.
DNA polymerase joins individual nucleotides to produce a DNA molecule and then “proofreads” each new DNA strand.
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Left Side Activity
Answer the following questions
What does DNA stand for?
What are the three components found in DNA?
What are the four bases found in DNA?
Explain base pairing.
Which bases pair with each other?
What is the structure of DNA?
Who discover that DNA had this structure?
What type of bonds connect the bases?
How many bonds are there between each of the two pairs of bases?
How does DNA replication occur?
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