1. Treatment EPO (1 IU/ml) Placebo
Hassouna et al Supplementary Figure 4:
EPO effects on quaternary E14 forebrain neurospheres cultured under proliferating and differentiating conditions A B
ICC
mRNA expression
Experimental design: Differentiating neurospheres
96h
168h
0.3h
24h
-0.3h
Seeding
Treatment EPO (1 IU/ml) Placebo C
Parameters and procedures D
Experimental design: Proliferating neurospheres
Dissociation of tertiary neurospheres
Parameters and procedures
Number by diameter
Treatment EPO (1 IU/ml) Placebo
Total number
144h E F G H