1. Estrogen enhancement of SGK1 expression induced by urocortin contributes to its cardioprotection against ischemia/reperfusion insult 
Binhai Cong, Jiankui Du, Xiaoyan Zhu, Jianqiang Lu, Xin Ni 
International Journal of Cardiology 
Volume 178, Pages (January 2015)
DOI: /j.ijcard
Copyright © 2014 Elsevier Ireland Ltd Terms and Conditions

2. Fig. 1
The effect of estrogen on SGK1 mRNA and protein expression in the myocardium and cardiomyocytes. A. Effect of Ovx and E2 replacement (Ovx-E2) on SGK1 expression in rat myocardium. E2 was administrated (30μg/kg/day, sc) for 8weeks. The mRNA and protein level of SGK1 in the myocardium was determined by real-time RT-PCR and Western blot, respectively. n=10 in each group. *P<0.05, **P<0.01 compared with sham; B–D. Effect of E2 on SGK1 expression in cardiomyocytes in vitro. B. Neonatal rat cardiomyocytes were treated with E2 (0.1–100nmol/L) for 24h. C. Cells were treated with E2 (10nmol/L) plus UCN1 (10nmol/L) for 24h. D. Cells were treated with E2 (10nmol/L) plus CRHR2 antagonist astrissin2B (Ast2B) (10nmol/L) under H/R for 24h. Four cultures (n=4) were performed in triplicate. *P<0.05, **P<0.01 compared with control; #P<0.05. Relative SGK1 was normalized to β-actin and presented as mean±SD. Representative Western blot bands are presented on the top of the corresponding graphs.
International Journal of Cardiology  , DOI: ( /j.ijcard )
Copyright © 2014 Elsevier Ireland Ltd Terms and Conditions

3. Fig. 2
SGK1 involves in the protective effects of E2 upon H/R injury in cardiomyocytes. Cardiomyocytes were transfected with control siRNA or SGK1 siRNA for 24h, and then treated with E2 (10nmol/L) under H/R for 24h. A. Overall cell viability assessed by MTT method; B. cell damage determined by supernatant LDH concentration; C. apoptosis assessed by cleaved caspase3 level in cardiomyocytes; relative cleaved caspase3 level was normalized to β-actin level and the representative Western blotting bands are on the top of corresponding graphs. All data are presented as mean±SD for four cultures (n=4) performed in triplicate. *P<0.05, **P<0.01.
International Journal of Cardiology  , DOI: ( /j.ijcard )
Copyright © 2014 Elsevier Ireland Ltd Terms and Conditions