1. Molecular Diagnosis of Cystoisosporiasis Using Extended-Range PCR Screening 
Sean C. Murphy, Daniel R. Hoogestraat, Dhruba J. SenGupta, Jennifer Prentice, Andrea Chakrapani, Brad T. Cookson 
The Journal of Molecular Diagnostics 
Volume 13, Issue 3, Pages (May 2011)
DOI: /j.jmoldx
Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2. Figure 1
Molecular diagnosis of cystoisosporiasis using molecular methods and retrospective confirmation by histologic examination. A: PCR-based detection of C. belli using primers directed against the 28S rRNA gene and ITS regions 1 and 2. PCR reactions were performed on extracted DNA, and PCR products were separated by electrophoresis using a 1% agarose gel and stained. Reaction products from primer sets are shown with the patient template (Pt), positive control (+), and negative control (−), as shown. A 100-bp DNA ladder is also shown. B: C. belli parasites demonstrated in the ileal mucosa in a biopsy specimen from the described patient. Multinucleated asexual schizonts (arrow), sexual macrogametocyte (wide arrowhead), and microgametocytes (narrow arrowheads) reside within enterocytes. The gastrointestinal epithelium demonstrated a normal-appearing epithelial layer with intact lamina propria lacking significant inflammation. Hematoxylin and eosin stain; original magnification ×40.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions