1. Recombinant H2 relaxin inhibits apoptosis and induces cell proliferation in cultured leiomyoma cells without affecting those in cultured normal myometrial cells 
Kaho Suzuki, M.D., Koji Nakabayashi, M.D., Ph.D., Ai Y. Yamada, M.D., Ph.D., Romana S.Z. Lodhi, M.D., Rhoichi Hazama, M.D., Ph.D., Yasuhiko Ebina, M.D., Ph.D., Hideto Yamada, M.D., Ph.D. 
Fertility and Sterility 
Volume 97, Issue 3, Pages (March 2012)
DOI: /j.fertnstert
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Immunocytochemical analysis of LGR7 in cultured human myometrial cells (A) and leiomyoma cells (C) after 72-hour subculture. Strong immunostaining of LGR7 in both myometrial (A) and leiomyoma cells (C) was observed. Replacement of the primary antibody with blocking peptide resulted in lack of positive immunostaining of LGR7 in both myometrial (B) and leiomyoma cells (D). Original magnification, ×40.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Effects of graded concentrations of relaxin on number of viable cultured leiomyoma cells and normal myometrial cells, as assessed by MTS assay. Cell viability in cultured leiomyoma cells (left) and myometrial cells (right) by 24- and 48-hour relaxin treatment were presented as fold increases over average number of viable cells with 24-hour untreated control cultures. Values represent the mean ± SE of at least three independent experiments. ∗P<.05, ∗∗P<.01 vs. untreated control cultures of each treatment.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
Effects of graded concentrations of rH2 relaxin on the PCNA-positive rate of cultured leiomyoma cells and normal myometrial cells. Proliferating cell nuclear antigen–positive rate in cultured leiomyoma cells (left) and myometrial cells (right) by 48-hour relaxin treatment. Values represent the mean ± SE of at least three independent experiments. ∗P<.01 vs. untreated control cultures.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

5. Figure 4
Effects of graded concentrations of relaxin on the TUNEL-positive rate (A) and caspase-3 expression (B) in cultured leiomyoma cells and normal myometrial cells. (A) The TUNEL-positive rate in cultured leiomyoma cells (left) and myometrial cells (right) by 48-hour relaxin treatment. (B) Caspase-3 expression in leiomyoma cells (left) and myometrial cells (right) cultured for 48 hour with relaxin and examined by Western blot analysis. Densitometric analysis of caspse-3 was also performed. Beta-actin was used to ensure the even loading of each specimen. Values represent the mean ± SE of at least three independent experiments. ∗P<.01, ∗P<.05 vs. untreated control cultures.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions