1. Bellwork: 10/25 Put phone up  Grab the papers from the front
Week 10/22-10/26
Grab the papers from the front
Grab your lab notebook
Take out your unit book

2. Objective
Students will learn about enzymes & set up a lab to apply their knowledge

3. Today:
Unit 2, pt. 8 Notes
Lab Notebook Set- Up
Mello Jello Pt. 1

4. This Week: Monday: Unit 2, pt. 5 & 6 notes
Tues/Wed: Unit 2, pt. 7 notes & macromolecules lab
Thursday: Unit 2, pt. 8 notes & Mello Jello Lab
Friday: Mello Jello Lab

5. Materials
Unit Book
Lab Notebook

6. Homework
None 

7. Unit II: Biochemistry

8. Part VII: Enzymes

9. Think:
What is a catalyst?

10. Intro Question
Someone says to you “you can eat as much as you want and never gain weight; you must have a high metabolism”. What does that statement mean biochemically?

11. Chemical Reaction
A process that changes one set of chemicals into another
This change comes from breaking and/or forming new chemical bonds
A + B -> C + D
Reactants Products

12. Chemical Reactions
Chemical reactions require an input of energy to start the reaction
Called ACTIVATION ENERGY
Chemical reactions would occur very slowly in living things due to insufficient Activation Energy

13. Catalysts
Speed up the chemical reactions by lowering activation energy
Not consumed by the reaction
Can be used over and over!
Biological Catalysts
Proteins called Enzymes

14. How Enzymes Work Folds to a specific shape, creating an Active Site
Substrate (Reactant) binds to Active Site
Chemical reaction occurs
Reactant converted to Product
Product is released from the enzyme
The free enzyme binds another substrate and starts the process over

15. Enzyme

16. Active Site
Shape of Active Site
Complementary to shape of Substrate

17. Lock and Key Model The grooves and prongs of the key
Must be complementary to grooves and prongs of the lock
Like the Active Site and the Substrate
Must be complementary

18. Environmental Effect
Extreme environments can affect enzyme shape & binding of the Substrate
2 key environmental factors
High or Low Temperature
pH too acidic or too basic
These environments cause the enzyme to denature (break down) by unfolding to its Primary Structure

19. Answer ?s in NG

20. Mello Jello

21. First… Date: 10/25/18 Lab Title: Mello Jello Lab Pages: ___-___
Table of Contents
Date Lab Title Pages
Date: 10/25/18
Lab Title: Mello Jello Lab
Pages: ___-___

22. Next…
10/25/18 #
Lab #: Mello Jello Lab
Page #
Lab Title

23. Next… # Group Members’ Names – same as yesterday!!!
10/25/18 #
Lab #: Mello Jello Lab
Group Names:
Group Members’ Names – same as yesterday!!!
Background Info – TAPE IN
Scientific ?
Hypothesis – “If…then..because…”
Variables
Independent
Dependent
Fixed Variables
Experimental Group
Control Group
Procedures – TAPE IN
Data Table – TAPE IN
Background Info: *Tape In*
Scientific Question:
Hypothesis:
Variables:
Independent:
Dependent:
Fixed:
Experimental Group:
Control Group:
Procedures (numbered):

24. Background Info
Foods are not only important nutrients for cells, they are also the cause of stains in clothes. Since some of the large complex molecules in food do not dissolve well in water, they are often left in clothes after washing. Enzymes are proteins that break down complex molecules in food to produce smaller molecules that are more soluble in water. For example, enzymes can break down the protein gelatin. These enzymes are known as proteases. They cleave proteins into individual amino acids by breaking the peptide bond. Manufacturers take advantage of the ability of proteases to break down food by adding them to laundry detergents to enhance stain removal and to meat tenderizers to make meat less tough.
In this lab, you will examine the effect of enzymes in detergents and other household cleaning products on jello.

25. Scientific Question Hypothesis
How do common household products that contain enzymes affect the structural integrity of jello?
If…then…because
Hypothesis

26. Variables Independent: Type of Liquids
Dependent: Change in well diameter
Fixed: Well size, amount of liquid used, environment
Experimental Group: Jello exposed to cleaners
Control Group: Well exposed to water

27. Data: #
Data:
Start on new page
Tape in!

28. Results: Tape in Graph Paper Bar or Line Graph? # # Results:
Summary of Results:

29. Conclusion (5-6 Sentences)
Reflection (5-6 Sentences) #
Conclusion:
Reflection:

30. *Tape in Analysis Questions*#
Analysis Questions: *Tape In*

31. Today: Lab Set-Up Get Jello petri dish
Label the dish (WRITE SMALL) ON THE TOP LID
One lab partner’s initials
Class Period

32. Today: Lab Set-Up
Cut wells in gelatin using a straw and a toothpick (BE CAREFUL)

33. Today: Lab Set-Up
4. Number the wells as according to your methods – BE CAREFUL!!

34. Today: Lab Set-Up
Measure and record the initial diameter of each well with a ruler (across each cutout)
Decide which solution goes in each well. Record.
DO NOT MIX DROPPERS

35. Today: Lab Set-Up Distilled water goes in #5
Carefully load the wells (follow methods carefully)
BE CAREFUL TO NOT SPILL LIQUIDS ANYWHERE ELSE ON PLATE
If you spill, mark it on your paper

36. Today: Lab Set-Up
When complete, put the lid back on the plate and leave on the table in the back room
Do NOT shake or tilt your plate

37. Tomorrow:
Take qualitative and quantitative observations/measurements of your plate

38. Tomorrow: Complete data table & calculations Complete graph
Begin writing down answers to the conclusion and analysis questions.