1. Supplemental Figure 1 20 40 60 80
100
% annexin V-stained cells
mean A B C D E F G H I J K L M N
% annexin V and 7AAD double-stained cells
untreated
Rituximab
mAb 37.1
mAb 37.2
Alemtuzumab
p =
p =
p =
p =
p =
p =
p =
p =
Supplemental Fig. 1 Antibody-induced direct cell death of CLL cells. After 24 h incubation in the presence of 10 µg / ml of each antibody, PS exposure and membrane integrity were determined by annexin V-FITC and 7AAD staining. The percentages of annexin V- and 7AAD-negative (top) or annexin V-negative cells (bottom) after treatment with 10 µg / ml rituximab, mAb37.1 and mAb37.2 or without treatment are shown for 14 individual samples. Letters refer to the patient information in Supplemental Table 1.