1. A nested polymerase chain reaction for detection of Legionella pneumophila in clinical specimens 
Sverker Bernander, Hanna-Stina Hanson, Bo Johansson, Lars-Victor von Stedingk 
Clinical Microbiology and Infection 
Volume 3, Issue 1, Pages (February 1997)
DOI: /j tb00257.x
Copyright © 1997 European Society of Clinical Infectious Diseases Terms and Conditions

2. Fig. 1
Agarose gel electrophoresis of nested PCR products. Lane 1: DNA molecular weight marker VIII (Boehringer Mannheim GmbH, Mannheim, Germany). Lanes 2, 4 and 6: three aliquots from the same DNA preparation of a sputum sample from a patient with Legionella infection. Lanes 3, 5, 7 and 10 are negative controls. Lane 8: a negative sputum. Lane 9: the same sample as in lane 8, but with 50 CFU of L. pneumophila sg 1 (ATCC 33152) added. Lane 11: positive control of heat-lysed L. pneumophila sg 1 (ATCC 33152).
Clinical Microbiology and Infection 1997 3, DOI: ( /j tb00257.x)
Copyright © 1997 European Society of Clinical Infectious Diseases Terms and Conditions