1. The Quid Pro Quo of the Tumor/Stromal Interaction
Mylène Tajan, Karen H. Vousden 
Cell Metabolism 
Volume 24, Issue 5, Pages (November 2016)
DOI: /j.cmet
Copyright © 2016 Elsevier Inc. Terms and Conditions

2. Figure 1
Metabolic Crosstalk between Ovarian Cancer Cells and Cancer-Associated Fibroblasts under Glutamine Starvation
Under conditions of glutamine starvation, cancer-associated fibroblasts (CAFs) show increased glutamine production (associated with increased aspartate and asparagine utilization) compared to normal fibroblasts, and co-culture with ovarian cancer cells further stimulates CAFs to secrete glutamine. Cancer cells can then use this glutamine as an anaplerotic substrate and a nitrogen donor for nucleotide synthesis, allowing them to grow under conditions of glutamine limitation. Cancer cells support glutamine synthesis in CAFs by stimulating their use of glucose and BCAAs, and by supplying them with lactate and glutamate. Co-targeting the ability of CAFs to produce glutamine by downregulating GS and the ability of cancer cells to catabolize glutamine by downregulating GLS results in a synergistic impairment of tumor growth in an orthotopic intra-ovarian mouse model. Asp, aspartate; Asn, asparagine; BCAAs, branched-chain amino acids; GLS, glutaminase; GS, glutamine synthetase; MCT1, monocarboxylate transporter 1.
Cell Metabolism  , DOI: ( /j.cmet )
Copyright © 2016 Elsevier Inc. Terms and Conditions