1. Fig. 1. NASH and fibrosis develop late in mice fed an HFD.
NASH and fibrosis develop late in mice fed an HFD. Mice were maintained on a normal diet (filled symbols; ND) or a 60% kcal from fat diet (open symbols; HFD) for 15 (circles) or 40 (squares) weeks. (A) Body weights and liver weight and liver index (% of body weight) were collected (n = 10 to 20). Characteristics of NASH were measured including isolated liver leukocyte counts (n = 9 to 12) (B), serum ALT and aspartate aminotransferase (AST) (n = 9 to 10) (C), and steatosis by Oil Red O staining (D). Scale bars, 100 μm. (E) Liver sections were stained with keratin 8/18 and ubiquitin (Ub.) to assess hepatocyte ballooning and hematoxylin and eosin (H&E) for hepatic inflammation (inset showing example of lobular inflammation). Scale bars, 40 μm. (F) Picrosirius red staining was performed to assess fibrotic collagen deposition (scale bars, 200 μm) and viewed under polarized light to enhance visualization (inset scale bars, 200 μm). Adipose inflammation was assessed from Giemsa-stained sections from 40-week–treated animals (scale bars, 150 μm) (G), crown-like structures marked with arrows, expression of tnf (H) (n = 4 to 5), and flow cytometry analysis of Siglec-F+ adipose eosinophils among CD45 leukocytes (I) (n = 5 to 12). All data points represent a single mouse, and representative or pooled data from two or more independent experiments are shown (two-tailed t tests, n = 2 to 10; *P < 0.05, **P < 0.01, ***P < 0.005, ****P < ).
Kevin M. Hart et al., Sci Transl Med 2017;9:eaal3694
Published by AAAS