1. Self Replicating Biochemical Systems
MAS.S62 FAB2
4.3.12
Self Replicating Biochemical Systems
Holliger et. al

2. What is the Threshold for Self Replicating Systems?
Threshold for Life
What is the Threshold for Self Replicating Systems?
Measurement Theory +
Step 1
Step 2
Step 3
Parts
Template
Machine
Replication Cycle
DNA
Error Correcting
Exonuclease
(Ruler)
Watson Crick
.18 nm
/sandwalk.blogspot.com/2007/12/dna-denaturation-and-renaturation-and.html
How Well Can N Molecules Measure Distance?
Probability of Self Replication
Threshold length: 691 bp for 50% yield bp for 10-6 yield.
Number of Nucleotides

3. 220 nt
Combinatorics
1:1E132
Library Diversity
1:1E15
Pligase = 1:2E13

4. Minimum ribozyme length 165 nt
14 nt extension
Error rate: 1%
Extension rate : 1nt per 153 minutes

6. Minimum ribozyme length:
20 nt extension
Error rate:
Extension rate :

8. 95 nt base extension