1. Containment Equipment and Facilities

2. Biocontainment The principle of holding or being capable of holding or including within a fixed limit or area The principle of holding or being capable of holding or including within a fixed limit or area Preventing the unintentional release of biological agents through a combination of laboratory practices, containment equipment (primary barrier) and laboratory facility design (secondary barrier) Preventing the unintentional release of biological agents through a combination of laboratory practices, containment equipment (primary barrier) and laboratory facility design (secondary barrier)

3. Primary Barrier Primary barriers contain the agent at the source Primary barriers contain the agent at the source Equipment/Engineering Control Equipment/Engineering Control Biological safety cabinet, fumehood, glove box, animal housing, centrifuge, fermenter Biological safety cabinet, fumehood, glove box, animal housing, centrifuge, fermenter

4. Secondary Barrier Secondary barrier is the structure surrounding the primary barrier Secondary barrier is the structure surrounding the primary barrier Facility/Engineering Control Facility/Engineering Control Rooms, building Rooms, building Types of Facilities Types of Facilities Basic laboratory Basic laboratory Containment laboratory Containment laboratory

5. Primary Barriers - Equipment Personnel Protection Personnel Protection Any aerosol generated within the cabinet is contained and kept away from the researcher Any aerosol generated within the cabinet is contained and kept away from the researcher Product Protection Product Protection Air within the work space of the cabinet has been filtered so that is is virtually free of airborne particles and organisms; thus protecting the work from outside contamination Air within the work space of the cabinet has been filtered so that is is virtually free of airborne particles and organisms; thus protecting the work from outside contamination Environmental Protection Environmental Protection Aerosols generated within the unit are removed from the air before the air is discharged Aerosols generated within the unit are removed from the air before the air is discharged

6. Ventilation Equipment Classes and Types

7. Chemical Fume Hood 100 fpm face velocity 100 fpm face velocity Offer only personnel protection Offer only personnel protection Always exhaust air to the outside Always exhaust air to the outside Do not offer protection to the product or the environment, as there is no filtration of intake and exhaust air (Sometimes air cleaning treatment is added to the exhaust.) Do not offer protection to the product or the environment, as there is no filtration of intake and exhaust air (Sometimes air cleaning treatment is added to the exhaust.) Do draw contaminants in the laboratory air directly over the product being worked on Do draw contaminants in the laboratory air directly over the product being worked on Used for work with chemical hazards Used for work with chemical hazards

8. Any Comments? Fumehood - keep hood clean, sash should be closed when hood is not in use, equipment should be 9 from sash

9. Clean Bench / Laminar Flow Hoods Provide product protection only Provide product protection only Product protection is provided by creating a unidirectional airflow generated through a HEPA filter Product protection is provided by creating a unidirectional airflow generated through a HEPA filter Discharge air goes directly into workroom Discharge air goes directly into workroom Applications Applications – Any application where the product is not hazardous but must be kept contaminant free – Preparation of non-hazardous intravenous mixtures and media – Particulate free assembly of sterile equipment and electronic devices Eliminate Clean Bench in containment laboratory Eliminate Clean Bench in containment laboratory

10. Biological Safety Cabinets Designed to contain biological hazards Designed to contain biological hazards Inward airflow for personnel protection Inward airflow for personnel protection HEPA filtered exhaust air for environmental protection HEPA filtered exhaust air for environmental protection Supply air HEPA filter for product protection (except Class I) Supply air HEPA filter for product protection (except Class I) Separated into Classes and Types Separated into Classes and Types – Class I – Class II Type A1, A2 Type A1, A2 Type B1, B2 Type B1, B2 – Class III Microbiological studies, cell cultures, pharmaceutical research and procedures… Microbiological studies, cell cultures, pharmaceutical research and procedures…

11. Class I Cabinet 75 fpm face velocity 75 fpm face velocity Provides personnel and environmental protection Provides personnel and environmental protection No product protection No product protection Requires an exhaust blower to pull the air through - usually to the outdoors Requires an exhaust blower to pull the air through - usually to the outdoors Applications Applications – Housing centrifuges, fermenters – Cage dumping in an animal lab – Aerating cultures

12. Class II Cabinets Ventilated cabinet Ventilated cabinet Provides personnel, product, and environmental protection Provides personnel, product, and environmental protection Open front with inward airflow for personnel protection Open front with inward airflow for personnel protection Downward HEPA filtered laminar airflow for product protection Downward HEPA filtered laminar airflow for product protection HEPA filtered exhaust air for environmental protection HEPA filtered exhaust air for environmental protection

13. Any Comments? BSC - remove unnecessary objects, keep grill at front of cabinet unobstructed

14. Primary Barriers PersonnelProductEnvironment Chemical Fumehood x Laminar Flowhood x Class I Biosafety Cabinet xx Class II Biosafety Cabinet xxx Class III Biosafety Cabinet xxx Isolatorsxxx

15. Types of Biosafety Cabinets NSF/ANSI Standard 49 – 2002 TypeFace velocity (lfpm) Airflow PatternRadionuclides/ Toxic Chemicals Bio- safety Level(s) Product Protection Class I75 In at front; rear and top through HEPA filter No2, 3No Class II Type A1 75 70% recirculated through HEPA; exhaust through HEPA No2, 3Yes Class II Type A2 100 30% recirculated through HEPA; exhaust via HEPA and hard ducted Yes (Low levels/volatility) 2, 3Yes Class II Type B1 100 No recirculation; total exhaust via HEPA and hard ducted Yes2, 3Yes Class II Type B2 100 Same as B1, but plena under negative pressure to room and exhaust air is ducted Yes2, 3Yes Class IIINA Supply air inlets and exhaust through 2 HEPA filters Yes3, 4Yes

16. Biological Safety Cabinet Certification First Certification First Certification Annually Annually When moved When moved When filter is changed When filter is changed When repaired or modified When repaired or modified Note: Certification is paid by the researcher, not EHS

17. Other Primary Barriers- Engineering Control Gasketed blenders, homogenizers Gasketed blenders, homogenizers Cotton plugs, filters for flasks in shakers Cotton plugs, filters for flasks in shakers Filtered pipette tips Filtered pipette tips HEPA and hydrophobic vacuum line filters HEPA and hydrophobic vacuum line filters Plasticware substituted for glassware Plasticware substituted for glassware Gas burners with shield, microincinerator Gas burners with shield, microincinerator Centrifuges Centrifuges Interlock, solid cover, safety buckets, O-rings Interlock, solid cover, safety buckets, O-rings

18. Secondary Barrier- Facilities Laboratory Biosafety Level 2 Lockable doors (a must for restricted agents) Lockable doors (a must for restricted agents) Sink Sink Bench tops impervious and easily cleaned Bench tops impervious and easily cleaned Biological safety cabinet (if applicable) Biological safety cabinet (if applicable) Eyewash Eyewash Inward airflow (desirable) Inward airflow (desirable)