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STATE DIFFUSION LAB Diffusion through a Membrane
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THE SUBSTANCE IS PRESENT
STATE DIFFUSION LAB NEGATIVE NO CHANGE IN COLOR Lt. BLUE POSITIVE CHANGE IN COLOR ORANGE/ BROWN Glucose Indicator BENEDICT’S SOLN: INDICATES (SHOWS YOU) THE SUBSTANCE IS PRESENT WHEN YOU SEE A CHANGE After heat
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STATE DIFFUSION LAB Table Two Chemical Test Results
Blue Colored Glucose Indicator ‘BENEDICT’S SOLN.’ ADDED TO dist. WATER BEFORE AFTER WAS THERE A CHANGE? NO IS GLUCOSE PRESENT? NO
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WAS THERE A CHANGE IN COLOR?
STATE DIFFUSION LAB Blue Colored Glucose Indicator ‘BENEDICT’S SOLN.’ ADDED TO STARCH BEFORE AFTER WAS THERE A CHANGE IN COLOR? NO IS GLUCOSE PRESENT? NO
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WAS THERE A CHANGE IN COLOR?
STATE DIFFUSION LAB AFTER HEAT Glucose Indicator ‘BENEDICT’S SOLN.’ ADDED TO GLUCOSE BEFORE HEAT WAS THERE A CHANGE IN COLOR? YES IS GLUCOSE PRESENT? YES
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STATE DIFFUSION LAB Solution ‘LUGOL’S IODINE’ INDICATES
POSITIVE CHANGE COLOR BLUE- BLACK Amber Colored Starch Indicator Solution ‘LUGOL’S IODINE’ INDICATES A STARCH IS PRESENT NEGATIVE NO CHANGE COLOR AMBER
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WAS THERE A CHANGE IN COLOR?
STATE DIFFUSION LAB Amber Colored Starch Indicator Solution ‘LUGOL’S IODINE’ INDICATES A STARCH IS PRESENT WATER before after WAS THERE A CHANGE IN COLOR? no IS STARCH PRESENT? no
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WAS THERE A CHANGE IN COLOR?
STATE DIFFUSION LAB Amber Colored Starch Indicator Solution ‘LUGOL’S IODINE’ INDICATES A STARCH IS PRESENT STARCH before after WAS THERE A CHANGE IN COLOR? YES YES IS STARCH PRESENT?
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WAS THERE A CHANGE IN COLOR?
STATE DIFFUSION LAB Amber Colored Starch Indicator Solution ‘LUGOL’S IODINE’ INDICATES A STARCH IS PRESENT GLUCOSE before after WAS THERE A CHANGE IN COLOR? no no IS STARCH PRESENT?
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‘BENEDICT’S SOLN.’ ADDED
STATE DIFFUSION LAB Glucose Indicator ‘BENEDICT’S SOLN.’ ADDED TO STARCH & GLUCOSE After heat OR Answer the question at the bottom of the page – What would you need to do to make this experiment a controlled experiment???
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‘BENEDICT’S SOLN.’ ADDED
STATE DIFFUSION LAB Glucose Indicator ‘BENEDICT’S SOLN.’ ADDED TO STARCH & GLUCOSE After heat OR BEFORE HEAT AFTER HEAT
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CELL IN WATER WITH IODINE
STATE DIFFUSION LAB CELL IN WATER CELL IN WATER WITH IODINE -INITIAL STATE 9. What do you think will happen to the substances inside & outside the “cell”?
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7. "CELL" IN WATER WITH IODINE
STATE DIFFUSION LAB Making the “Cell” Read Steps 1-5 2.Add Glucose & Starch to"CELL" 6."CELL" IN WATER 7. "CELL" IN WATER WITH IODINE -INITIAL STATE
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2. CELL IN WATER WITH IODINE
STATE DIFFUSION LAB 1. CELL IN WATER 2. CELL IN WATER WITH IODINE -INITIAL STATE 3. AFTER TIME -FINAL STATE
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2. CELL IN WATER WITH IODINE
STATE DIFFUSION LAB 1. CELL IN WATER 2. CELL IN WATER WITH IODINE -INITIAL STATE 3. AFTER TIME -FINAL STATE
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STATE DIFFUSION LAB 2. CELL IN WATER WITH IODINE -INITIAL STATE
Model Cell Observations Record any changes you see TESTING BEAKER SOLUTION 2. CELL IN WATER WITH IODINE -INITIAL STATE 1. CELL IN WATER 3. AFTER TIME -FINAL STATE
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‘BENEDICT’S SOLN.’ ADDED
STATE DIFFUSION LAB Glucose Indicator ‘BENEDICT’S SOLN.’ ADDED TO Beaker Solution 3. AFTER TIME -FINAL STATE After heat OR Answer Questions 1-7
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STATE DIFFUSION LAB Part Two –
Diffusion of Water Across a Membrane ( Osmosis ) Using Red Onion Cells Reading and steps 1-5
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1.Snap onion slide 2.Peel 3. Wet Mount
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6. Red Onion cell in water 7. Now add Salt Solution
State Diffusion Lab Cell Wall Cell membrane Cytoplasm Normal Red Onion Cell 6. Red Onion cell in water 7. Now add Salt Solution
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Add several drops of salt solution to one side of the cover slip
State Diffusion Lab Add several drops of salt solution to one side of the cover slip While placing a small piece of paper towel along the opposite edge of the cover slip The paper should draw out the water and draw in the salt solution Make sure that the salt solution has time to diffuse under the cover slip salt solution salt solution paper towel paper towel
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State Diffusion Lab Cell Wall Cell membrane Cytoplasm Cell Membrane Cytoplasm Normal Red Onion Cell Red Onion in Salt Water What happens to a red onion cell when you add saline solution (salt water) to it?
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QUESTIONS 12. 15. Red onion cell Red onion cell in salt solution
What makes the cell membrane go back to normal? Red onion cell Red onion cell in salt solution Add Distilled Water (Pure Water) Distilled water makes a cell GROW the most QUESTIONS
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State Diffusion Lab Answer analysis questions to complete the lab
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