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The Haystack Is Full of Needles: Technology Rescues Sugars!

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1 The Haystack Is Full of Needles: Technology Rescues Sugars!
Richard D. Cummings  Molecular Cell  Volume 68, Issue 5, Pages (December 2017) DOI: /j.molcel Copyright © 2017 Elsevier Inc. Terms and Conditions

2 Figure 1 The General Flow Chart for Glycoproteomics Using the Approach Described by Stadlmann et al. (2017) The proteins and glycoproteins from cells are digested by protease, and glycopeptides are enriched by chromatography and identified for sequence, glycosylation site, and glycan composition/structure through MS and computational approaches. MS, mass spectrometry; nLC, nano-liquid chromatography; HILIC, hydrophilic interaction chromatography; ESI, electrospray ionization; HCD, higher energy collisional dissociation; PNGase F, peptide-N-glycosidase F. Molecular Cell  , DOI: ( /j.molcel ) Copyright © 2017 Elsevier Inc. Terms and Conditions

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