1. Material Safety Data Sheets Hazards in the Lab Chemical Classes
Biotech Lab Safety
Material Safety Data Sheets
Hazards in the Lab
Chemical Classes

2. Why is safety and safety training important for laboratory work?
Bell Work
Answer the following question in your lab notebook:
Why is safety and safety training important for laboratory work?

3. Objectives
Identify specific biological/biohazardous/ chemical materials. (1.7)
Students will locate the material safety data sheets for the facility, and identify appropriate internet MSDS resources.
Students will create a safety map of the facility.
Students will apply knowledge of material safety data sheets (MSDS) (1.3)

4. Key Terminology Material Safety Data Sheet (MSDS) Biohazard
A communication from a chemical’s manufacturer that contains vital information regarding safe use and disposal of the chemical and its properties.
Biohazard
A substance that poses a danger to life/living things.
Safety Equipment GROUP Definition and Sort

5. Safety Equipment With your table group: Based on our class definition:
Define Safety Equipment on your whiteboard.
Be prepared to share your table’s definition.
Based on our class definition:
Sort the Deck into “Safety” and “Non-Safety” groups.

6. Introduction
Science may be hazardous, but can be done safely when hazards are controlled.
Everyone is responsible: students, teachers, supervisors, and higher administrators.
All safety information brought to you today applies to all science disciplines.
Remember - each discipline has SPECIFIC and unique information and safety protocols.

7. Hazards in the Lab: Biological Controls
You will encounter biological materials throughout this course.
Wear proper Personal Protective Equipment
Universal Precautions: Treat everything like it is infectious.
Disinfect your lab bench each day:
School provided disinfectant spray
Freshly prepared solution of 1 part household bleach and 10 parts water
70% Ethanol(alcohol)

8. Hazards in the Lab: Biological Hazards
What could be encountered?
Bacteria, fungi/mold
Injured student/ faculty
Human blood sampling, tissues
(not common in high school labs)

9. Hazards in the Lab? Where do you start?
Gather Standard Operating Procedures and other information for your facility
Material Safety Data Sheets

10. Material Safety Data Sheet
Here is the MSDS for Sodium chloride (NaCl)
What sections are found on an MSDS?
1. Paste the MSDS into your Lab Notebook
2. Highlight the sections listed on an MSDS
Section 1 = ?
Section 2 = ?
Etc.

11. Material Safety Data Sheets (MSDS)
Contain all safety and use information about a chemical.
Manufacturers are required to provide them when chemicals are purchased.
MSDS are an important part of OSHA Hazard Communication Standard ( )
MSDS must be readily available to employees.
Be sure to read all sections before using a chemical.

12. MSDS Activity Sheet Using the MSDS Activity Sheet:
Think about the answers on the worksheet
Pair up, and SHARE with your neighbor what the answers would be….don’t write anything yet!

13. Where’s the MSDS?
Material Safety Data Sheets are required to be present in ANY facility that uses a chemical.
Where are ours?
Record the location in your lab notebook
You can even take a picture with your digital camera or phone if the teacher says yes, and paste that in your lab notebook

14. Find One!
Using the internet, or one of the MSDS Binders, locate an MSDS for a chemical used in our facility.
Some chemicals used in our facility are:
Copper Sulfate Pentahydrate
Sodium Hydroxide
LB Agar
Acetic Acid
LB Broth
TAE Buffer
Ethidium Bromide
Crystal Violet
Ethanol
Glucose
Click MSDS Search

15. Thumbs Up-Thumbs Down
Was the MSDS binder in an easily accessible location?

16. Hazards in the Lab: Chemical Classes
Chemicals are segregated according to class:
Information about Toxicity, oxidation, etc are identified on the MSDS
Acids
Bases
Solvents
Flammables
Halogenated
Pyrophoric
Peroxide Forming
Toxic
Oxidizers

17. Chemical Classes: Acids
Def: The pH range of acids is from
Examples: hydrochloric acid (HCl), acetic acid, nitric acid, phenol, sulfuric acid
Storage Precautions:
Store in large bottles of acids on low shelf or in acid cabinets.
Segregate acids from bases.
Have spill kit with neutralizers and absorbent.

18. Proper Storage for Acids:

19. Inside Look at Acid Storage:

20. Improper Acid Storage
Rust

21. Chemical Classes: Bases
Def: The pH range of basic solutions is from
Examples: sodium hydroxide (NaOH), ammonium hydroxide,
Storage Precautions:
Segregate bases from acids
Have a spill kit with neutralizers and absorbent.

22. Chemical Classes: Flammables
Def: Has a flash point of 60.5°C (141°F) or lower
Examples: acetone, ethanol, methanol, isopropyl alcohol
Storage Precautions:
Store in approved safety cans or cabinets
Segregate from oxidizing acids and oxidizers
Keep away from any source of ignition: flames, localized heat, or sparks.
Store highly volatile flammable liquids in a specially equipped refrigerator.

23. Example of Proper Storage: Flammables

24. Chemical Classes: Pyrophoric
Def: A material which ignites spontaneously upon exposure to air (or oxygen).
Examples: Powders: calcium, zinc, lead, iron, nickel
Storage Precautions: Store sealed in a cool, dry place

25. Chemical Classes: Peroxide-Forming Chemicals
Def: Organic compounds that spontaneously form peroxides by a free-radical reaction with molecular oxygen in a process of auto-oxidation.
Examples: ethyl ether, isopropyl ether, acetaldehyde
Storage Precautions:
Store in airtight containers in dark, cool, dry place.
Label containers with receiving, opening, and disposal dates.
Dispose of peroxide-forming chemicals before expected date of first peroxide formation.
Test for presence of peroxides periodically.

26. Chemical Classes: Toxic
Def: Dangerous or extremely dangerous to health and life when inhaled, swallowed, or absorbed by skin contact.
Examples: phenol, arsenic, chloroform, nitric acid, sulfuric acid, hydrogen peroxide, hydrochloric acid
Storage Precautions:
Store according to hazardous nature of chemical, using appropriate security when necessary.
Turn to your partner – Where would you find the information about how to store the chemical?

27. Find The Safety Equipment!
Take a quick tour of your facility!
Locate and record in your lab notebook the location(s) of:
Hazards in the Lab (biohazards or chemical)
Safety Equipment
Chemical Storage Locations (look for labeled, specialty cabinets and standard cabinets with chemicals)

28. Chemical Hazards Thumbs Up-Thumbs Down
Key issue in use, storage, and disposal is:
CHEMICAL COMPATIBILITY!!
Thumbs Up-Thumbs Down
Is chemical compatibility information on an MSDS?

29. Chemical Compatibility Chart

30. Closure Why are material safety data sheets required in a facility?
Take one minute to THINK, then WRITE for one minute about why facilities require MSDS, and why YOU as a technician should read them often.

31. Due tomorrow at the beginning of class.
Homework
Complete the MSDS Activity Sheet using the MSDS you found. If an electronic version is used, you DO NOT need to print it, but you do have to record your source website.
Identify at least 3 hazards in your facility.
Describe them, and determine ways to prevent or minimize them.
Due tomorrow at the beginning of class.

32. References
Roy, Ken; Safe Science Series June 09, 2011.
Lab Safety
June 9, 2011
June 9, 2011.
Chemical Safety
“Chemical Safety for Teachers and Their Supervisors- Grades 7-12”, A publication of the American Chemical Society and the ACS Board-Council Committee on Chemical Safety. Send to for a copy.
MSDS
June 9, 2011
Click MSDS Search June 10, 2011