1. LAB 3
ENZYMATIC DIGESTION

2. OBJECTIVES BUILDING BLOCKS OF PROTEINS, FATS, AND CARBOHYDRATES
ENZYMES RESPONSIBLE FOR THE HYDROLYSIS OF PROTEINS, FATS, AND CARBOHYDRATES
TEST USED TO DETERMINE IF HYDROLYSIS OF THESE MOLECULES HAVE OCCURRED.

3. BIOMOLECULES 4 major classes of biomolecules 1. Carbohydrates
2. Proteins
3. Lipids
4. Nucleic Acid
These biomolecules are made by dehydration and they are broken apart by hydrolysis.
There is a specific enzyme to catalyze each reaction

4. PROCESS OF DIGESTION
Mechanical digestion – chewing, mixing, and peristalsis
Chemical digestion – special enzymes
Hydrolysis – this is added to help in the breakdown of larger food molecules into smaller molecules

5. Type of digestion --- Tube contents Carbohydrate Digestion
TEST THAT WILL PERFORM IN LAB TO TEST FOR THE PRESENCE OF SMALLER MOLECULES IN THE PROCESS OF DIGESTION
Type of digestion --- Tube contents
Protein Digestion
Lipid Digestion
Carbohydrate Digestion
Substrate (Reactant)
Albumin
Olive oil
Starch
Enzyme
Trypsin
Pancreatic lipase
Amylase
Expected product
Amino acids
Glycerol and fatty acids
Maltose
Assay for substrate
Biuret test
+ violet
+/ - pink
pH test
+ decrease in pH
-No pH change
Iodine test
+ blue/ black
-Brown/ yellow
Assay for product
Ninhydrin test
+ blue/ violet
-colorless
Benedict’s test
+ red/ orange
+/ - yellow/ green
-blue