1. Experimental esophagitis induced by acid and pepsin in rabbits mimicking human reflux esophagitis 
Angel Lanas*, Yolanda Royo‡, Javier Ortego§, M. Molina*, R. Sáinz* 
Gastroenterology 
Volume 116, Issue 1, Pages (January 1999)
DOI: /S (99)
Copyright © 1999 American Gastroenterological Association Terms and Conditions

2. Fig. 1
Surgical procedure used to perfuse AP into the esophageal lumen. Rabbits underwent surgery for placement of a plastic tube (internal diameter, 5 mm) into the lumen of the cervical esophagus. The tube was secured with surgical ligatures, tunneled, and fixed through the skin on the posterior cervical area, allowing free access to the lumen of the esophagus.
Gastroenterology  , DOI: ( /S (99) )
Copyright © 1999 American Gastroenterological Association Terms and Conditions

3. Fig. 2
Microphotograph of immunohistochemical staining with the antibody mib1, which detects the Ki67 nuclear antigen of proliferation, showing more than 5 rows of proliferating cells at the basal level.
Gastroenterology  , DOI: ( /S (99) )
Copyright © 1999 American Gastroenterological Association Terms and Conditions

4. Fig. 3
Macroscopic and microscopic scores of mucosal damage with the different perfusion regimens of 60, 45, and 30 minutes at days (A) 3 and (B) 5 (**P < vs. other regimens; *P < 0.05 vs. 30 min/12 h).
Gastroenterology  , DOI: ( /S (99) )
Copyright © 1999 American Gastroenterological Association Terms and Conditions

5. Fig. 3
Macroscopic and microscopic scores of mucosal damage with the different perfusion regimens of 60, 45, and 30 minutes at days (A) 3 and (B) 5 (**P < vs. other regimens; *P < 0.05 vs. 30 min/12 h).
Gastroenterology  , DOI: ( /S (99) )
Copyright © 1999 American Gastroenterological Association Terms and Conditions

6. Fig. 4
Macroscopic and microscopic scores of mucosal damage with the different perfusion regimens (60, 45, and 30 minutes) at different time intervals. (A) **P ≤ 0.01 vs. all others; φP < 0.05 vs. all microscopic scores and macroscopic scores of the 60 min/12 h regimen. (B) **P < 0.01, *P < 0.05 vs. day 1. (C) **P < 0.01, *P < 0.05 vs. days 1, 5, 7, and 20.
Gastroenterology  , DOI: ( /S (99) )
Copyright © 1999 American Gastroenterological Association Terms and Conditions

7. Fig. 4
Macroscopic and microscopic scores of mucosal damage with the different perfusion regimens (60, 45, and 30 minutes) at different time intervals. (A) **P ≤ 0.01 vs. all others; φP < 0.05 vs. all microscopic scores and macroscopic scores of the 60 min/12 h regimen. (B) **P < 0.01, *P < 0.05 vs. day 1. (C) **P < 0.01, *P < 0.05 vs. days 1, 5, 7, and 20.
Gastroenterology  , DOI: ( /S (99) )
Copyright © 1999 American Gastroenterological Association Terms and Conditions

8. Fig. 4
Macroscopic and microscopic scores of mucosal damage with the different perfusion regimens (60, 45, and 30 minutes) at different time intervals. (A) **P ≤ 0.01 vs. all others; φP < 0.05 vs. all microscopic scores and macroscopic scores of the 60 min/12 h regimen. (B) **P < 0.01, *P < 0.05 vs. day 1. (C) **P < 0.01, *P < 0.05 vs. days 1, 5, 7, and 20.
Gastroenterology  , DOI: ( /S (99) )
Copyright © 1999 American Gastroenterological Association Terms and Conditions

9. Fig. 5
Microphotographs of mild esophageal damage (low-grade esophagitis) obtained after perfusion of AP for 30 min/12 h for 7 days (H&E stain). (A) Superficial epithelial loss, basal hyperplasia, and extended papillae. (B) Extended papillae, basal hyperplasia, vasodilatation, and edema of the submucosa.
Gastroenterology  , DOI: ( /S (99) )
Copyright © 1999 American Gastroenterological Association Terms and Conditions

10. Fig. 6
Macroscopic appearance of formaldehyde-fixed esophageal specimens. (A) Mild esophagitis showing edema, hyperemia, petechia, and focal and isolated areas of submucosal hemorrhage. (B) Severe esophagitis with extensive longitudinal erosion, ulceration, and bleeding of the lower and middle esophagus. (C) Severe esophageal damage with longitudinal and confluent ulceration of the middle esophagus.
Gastroenterology  , DOI: ( /S (99) )
Copyright © 1999 American Gastroenterological Association Terms and Conditions

11. Fig. 7
Microphotographs of severe esophageal damage (high-grade esophagitis) obtained after perfusion of AP for 45 min/12 h for 5 days (H&E stain). (A) Extensive epithelial loss with intense edema and infiltration of inflammatory cells (polymorphonuclear and lymphocyte) in the mucosa and submucosa. (B) Details of specimens with ulceration and moderate infiltration of inflammatory cells. (C) Other areas with extended papillae, edema, and bleeding.
Gastroenterology  , DOI: ( /S (99) )
Copyright © 1999 American Gastroenterological Association Terms and Conditions