1. Cytoplasm replacement following germinal vesicle transfer restores meiotic maturation and spindle assembly in meiotically arrested oocytes 
John Zhang, Hui Liu 
Reproductive BioMedicine Online 
Volume 31, Issue 1, Pages (July 2015)
DOI: /j.rbmo
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2. Figure 1
Schematic diagram of GV transfer between IBMX-treated oocyte and a control non-treated oocyte.
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3. Figure 2
The effect of IBMX arrest on meiotic spindle in mouse oocytes. Indirect immunofluorescent staining for β-tubulin (A, B, C, D, E); Hoechst staining for chromosomes (a, b, c, d, e). Normal meiotic spindle observed in control metaphase II oocytes (A and a). Oocytes arrested with IBMX for 6 h were similar to controls (not shown). Abnormal meiotic spindle observed in metaphase II oocytes arrested with IBMX for 48 h (B, b, C, c, D, d, and E, e).
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4. Figure 3
Effect of cytoplasm replacement by GV transfer on meiotic spindle of mouse oocytes arrested by IBMX. Indirect immunofluorescent staining for β-tubulin (A, B, C, D, E); Hoechst staining for chromosomes (a, b, c, d, e). Abnormal meiotic spindle observed in oocytes reconstructed with GV/CIBMX (A, a, B, b, C, c, D, d). Normal meiotic spindle observed in oocytes constructed with GVIBMX/C (E, e). C = cytoplasm; GV = germinal vesicle.
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5. Figure 4
Birefringent spindle observed in H/M and M/H types of xeno-oocytes at metaphase II. Metaphase II oocytes of human (A, a) and mouse (B, b) as well as metaphase II reconstituted xeno-oocytes of M/H (C, c) and H/M (D, d). Birefringent spindle in metaphase II oocytes of human (a), mouse (b), and in metaphase II reconstituted xeno-oocytes of M/H (c) and H/M (d). GV = germinal vesicle; H/M = oocyte reconstituted by human GV and mouse cytoplasm; M/H = oocyte reconstituted by mouse GV and human cytoplasm.
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6. Figure 5
Cytogenetic analysis of mature H/M and M/H xeno-oocytes. Twentry-three sets of univalent chromosomes found in metaphase II H/M xeno-oocytes (A). Premature division of chromatids detected in metaphase II M/H xeno-oocyte (B). GV = germinal vesicle; H/M = oocyte reconstituted by human GV and mouse cytoplasm; M/H = oocyte reconstituted by mouse GV and human cytoplasm.
Reproductive BioMedicine Online  , 71-78DOI: ( /j.rbmo )
Copyright © 2015 Reproductive Healthcare Ltd. Terms and Conditions