1. MTHFR-Ala222Val and male infertility: a study in Iranian men, an updated meta-analysis and an in silico-analysis 
Hossein Nikzad, Mohammad Karimian, Kobra Sareban, Maryam Khoshsokhan, Abasalt Hosseinzadeh Colagar 
Reproductive BioMedicine Online 
Volume 31, Issue 5, Pages (November 2015)
DOI: /j.rbmo
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2. Figure 1
Human MTHFR gene map and Ala222Val (677C>T) genotyping: (A) human MTHFR gene map was deduced from the National Center for Biotechnology Information data bank, which contains 12 exon; the location of 677C>T on Hinf1 restriction site shown by arrow. F and R represent forward and reverse primers, respectively; (B) polymerase chain reaction (PCR) product of exon5 (400-bp) containing Ala222Val [lane M = 100-bp DNA ladder; lanes 1–3 = PCR product containing Ala222Val]; (C) enzymatic digestion results for MTHFR-Ala222Val polymorphism. Digestion map for 400-bp PCR fragment by Hinf1 enzyme [lane M = DNA marker; lane1 = the sample with Ala/Ala genotype containing a 400-bp uncut band; lane2 = the sample with Ala/Val genotype containing 400-bp, 250-bp and 150-bp bands; lane 3 = the sample with Val/Val genotype containing 250-bp and 150-bp bands]; (D) partial sequences of MTHFR gene at the Ala222Val polymorphism location: the samples with Val/Val (or CC) genotype, Ala/Val (or CT) genotype and Val/Val (or TT) genotype. The polymorphic site is shown by arrows.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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3. Figure 2 Results of the search strategy and the reasons for exclusion.
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4. Figure 3
Meta-analysis for 23 studies of Ala222Val: (A) allelic model; (B) codominant model.
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5. Figure 4
Meta-analysis for 23 studies of Ala222Val: (A) dominant model; (B) recessive model.
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6. Figure 5
Ramachandran plot, partial hydrophobicity pattern of MTHFR-protein and mRNA structures: (A) Ramachandran plot for 222Ala allele (left) and 222Val allele (right), the location of residue222 shown by arrows (Phi: involving the backbone atoms C′-N-Cα-C′, and Psi: involving the backbone atoms N-Cα-C′-N); (B) average hydrophobicity is 0.5 for Ala222 (right) and around 1 for Val222 (left). The residue222 is shown by a blue dot; (C) the secondary structure of wild-type mRNA (right) and mutant mRNA (left). Two loops removed in secondary structure of global mutant are compared with the normal type. The changes are shown by arrows. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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