1. Nature biotechnology, 20(2) 177-182
An unnatural base pair for incorporating amino acid analogs into proteins
Ichiro Hirao et al.
Nature biotechnology, 20(2)
MEC Seminar
Su Dong Kim

2. Unnatural base pair of s-y expands the genetic code
s : 2-amino-6-
(2-thienyl)
purine
y : pyridin-2-one
yAG codon
ClTyr
: Chlorotyrosine
ras protein

3. Fig. 1 The unnatural x-y (A) and s-y (C),
and noncognate x-T (B) and s-T (D) base pairs

4. Fig. 2
T7 transcription employing s-y pairing

5. Fig. 3
The transcription-translation system involving the s-y base pair

6. Fig. 4
Preparation of aminoacylated tRNACUs

7. Fig. 5a Autoradiogram of in vitro transcription-translation products
labeled with I-[14C] leucine

8. Fig. 5b
LC patterns of the products digested by Lys-C

9. Fig. 5c
Tandem mass spectrum of F-cY32 fragment