1. Microscope
Basics
T. Trimpe

2. Eyepiece (Ocular lens) Contains a 10x lens Body Tube B. K. C. E. D. J.
(not on sheet) B. K. C. E. D. J. H. F. I.
Always carry a microscope with one hand holding the arm and one hand under the base. G.

3. Holds the objective lenses, rotates.
Eyepiece
(Ocular lens)
Body Tube
(not on sheet)
B. Nosepiece
Holds the objective lenses, rotates. K. E. D. J. H. F. I.
Always carry a microscope with one hand holding the arm and one hand under the base. G.

4. Eyepiece (Ocular lens) Body Tube B. Nosepiece K. C. Objective lenses
(not on sheet)
B. Nosepiece K.
C. Objective lenses
3 choices of power: 4x, 10x, 40x E. J. H. F. I.
Always carry a microscope with one hand holding the arm and one hand under the base. G.

5. D. Stage clips hold the slide in place.
Eyepiece
(Ocular lens)
Body Tube
(not on sheet)
B. Nosepiece K.
C. Objective lenses
D. Stage clips hold the slide in place. E. J. H. F. I.
Always carry a microscope with one hand holding the arm and one hand under the base. G.

6. Controls amount of LIGHT. I.
Eyepiece
(Ocular lens)
Body Tube
(not on sheet)
B. Nosepiece K.
C. Objective lenses E.
D. Stage clips J.
H. Diaphragm
Controls amount of LIGHT. I.
Always carry a microscope with one hand holding the arm and one hand under the base. G.

7. Shines light up through specimen.
Eyepiece
(Ocular lens)
Body Tube
(not on sheet)
B. Nosepiece K.
C. Objective lenses E.
D. Stage clips J.
H. Diaphragm
F. Light Source
Shines light up through specimen. I.
Always carry a microscope with one hand holding the arm and one hand under the base. G.

8. Eyepiece (Ocular lens) Body Tube B. Nosepiece K. C. Objective lenses
(not on sheet)
B. Nosepiece K.
C. Objective lenses E.
D. Stage clips J.
H. Diaphragm
F. Light Source I.
Always carry a microscope with one hand holding the arm and one hand under the base.
G. Base

9. Eyepiece (Ocular lens) Body Tube B. Nosepiece K. C. Objective lenses
(not on sheet)
B. Nosepiece K.
C. Objective lenses E.
D. Stage clips J.
H. Diaphragm
F. Light Source I.
Always carry a microscope with one hand holding the arm and one hand under the base.
G. Base
Supports all

10. I. Fine Adjustment Knob slightly moves the stage to SHARPEN image.
Eyepiece
(Ocular lens)
Body Tube
(not on sheet)
B. Nosepiece K.
C. Objective lenses E.
D. Stage clips J.
H. Diaphragm
I. Fine Adjustment Knob slightly moves the stage to SHARPEN image.
F. Light Source
Always carry a microscope with one hand holding the arm and one hand under the base.
G. Base

11. Eyepiece (Ocular lens) Body Tube B. Nosepiece K. C. Objective lenses
(not on sheet)
B. Nosepiece K.
C. Objective lenses E.
D. Stage clips
J. Coarse Adjustment Knob Moves stage up & down. Use only in 4x or 10x, not 40x!
H. Diaphragm
F. Light Source
Always carry a microscope with one hand holding the arm and one hand under the base.
G. Base

12. Eyepiece (Ocular lens) Body Tube B. Nosepiece K. C. Objective lenses
(not on sheet)
B. Nosepiece K.
C. Objective lenses
E. Stage
Supports the slide.
D. Stage clips
H. Diaphragm
I. Fine Adjustment Knob
F. Light Source
Always carry a microscope with one hand holding the arm and one hand under the base.
G. Base

13. Used to carry, holds nosepiece & eyepiece. B. Nosepiece
(Ocular lens)
Body Tube
(not on sheet)
K. Arm
Used to carry, holds nosepiece & eyepiece.
B. Nosepiece
C. Objective lenses
D. Stage clips
J. Coarse Adj. Knob
H. Diaphragm
I. Fine Adjustment Knob
F. Light Source
Always carry a microscope with one hand holding the arm and one hand under the base.
G. Base

14. Eyepiece (Ocular lens) Body Tube B. Nosepiece K. Arm
(not on sheet)
B. Nosepiece
K. Arm
C. Objective lenses
E. Stage
D. Stage Clips
J. Coarse Adjustment
H. Diaphragm
F. Light Source
I. Fine Adjustment
Always carry a microscope with one hand holding the arm and one hand under the base.
G. Base

15. What’s my power?
To calculate the power of magnification, multiply the power of the ocular lens by the power of the objective.
What are the powers of magnification for each of the objectives we have on our microscopes?
Example: When using your 40x objective lens, and looking through the 10x ocular lens, what you are looking at is magnified to appear 400 times (40 x 10) its actual size!

16. Look at your lenses…
The ocular lens says: _____x
The Objective lenses:
Low= ______x
Medium = ______x
High = ______x

17. Comparing Powers of Magnification
We can see better details with higher the powers of magnification, but we cannot see as much of the image.
(LIKE “ZOOMING IN”)
Which of these images would be viewed at a higher power of magnification? A B

18. Let’s give it a try ...
1 – Turn on the microscope and then rotate the nosepiece to click the red-banded objective into place.
2 – Place a slide on the stage and secure it using the stage clips. Use the coarse adjustment knob (large knob) to get it the image into view and then use the fine adjustment knob (small knob) to make it clearer.
3 – Once you have the image in view, rotate the nosepiece to view it under different powers. Draw what you see on your worksheet!
Be careful with the largest objective! Sometimes there is not enough room and you will not be able to use it!
4 – When you are done, turn off the microscope and put up the slides you used.

19. You do not need to use the stage clips when viewing wet-mount slides!
How to make a wet-mount slide …
1 – Get a clean slide and coverslip from your teacher.
2 – Place ONE drop of water in the middle of the slide. Don’t use too much or the water will run off the edge and make a mess!
3 – Place the edge of the cover slip on one side of the water drop.
4 - Slowly lower the cover slip on top of the drop.
Cover Slip
Lower slowly
You do not need to use the stage clips when viewing wet-mount slides!
5 – Place the slide on the stage and view it first with the red-banded objective. Once you see the image, you can rotate the nosepiece to view the slide with the different objectives.