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Volume 127, Issue 5, Pages 1356-1371 (November 2004)
Occult hepatitis B virus infection in chronic liver disease: Full-length genome and analysis of mutant surface promoter Vaishali Chaudhuri, Ruchi Tayal, Baibaswata Nayak, Subrat Kumar Acharya, Subrat Kumar Panda Gastroenterology Volume 127, Issue 5, Pages (November 2004) DOI: /j.gastro Copyright © 2004 American Gastroenterological Association Terms and Conditions
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Figure 1 (A) Lane M, ζ Hind III marker; lane 1, positive (HBsAg-/HBeAg-positive) control; lane 2, negative control; lanes 3–6, HBsAg-positive samples. (B) Lane M, ζ Hind III marker; lane 1, negative control; lane 2, HBsAg-positive control; lane 3, positive (HBsAg-/HBeAg-positive) control; lanes 4–7, S2, S3, S4, and S9. Gastroenterology , DOI: ( /j.gastro ) Copyright © 2004 American Gastroenterological Association Terms and Conditions
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Figure 2 Phylogenetic analysis of variants of S1-S9 and SWT.1-SWT.5 with genotype A and D isolates from Genbank. Gastroenterology , DOI: ( /j.gastro ) Copyright © 2004 American Gastroenterological Association Terms and Conditions
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Figure 3 Schematic representation showing types of variations in the pre-S1-S2 region affecting the S promoter. Top, the essential S-promoter region (□), transcription factor binding sites (■), B-cell epitopes and start sites of the pre S1/S2/S ATG (•). Regions corresponding to wild type in all variants are shown by a continuous line, deletions are shown by a textured pattern box, and changes in reading frame are shown by slashes. Gastroenterology , DOI: ( /j.gastro ) Copyright © 2004 American Gastroenterological Association Terms and Conditions
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Figure 4 Schematic representation of variations observed in the precore/core region. Start sites of precore and pregenome RNA is indicated by arrows. Sites for DR1 and RNA encapsidation are marked. Codons corresponding to wild type are marked above the continuous line, representing the precore/core open reading frame. Corresponding mutation in the variants (S1-S9) are marked on the lower part. Gastroenterology , DOI: ( /j.gastro ) Copyright © 2004 American Gastroenterological Association Terms and Conditions
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Figure 5 Comparative analysis of the expression levels of the rLUC gene from HBsAg-rLUC fusion constructs in promoterless vector pRLnull. The wild-type (ayw3 subtype) and variants S1 (nt. ▵ ), S3.2 (▵ nt ), S3.3 (▵ nt ), S5.1 (▵ nt. 3025–3208), and S8.1 (▵ nt. 3034–3090) were analyzed. The experiments were equalized by cotransfection with pSG-fLUC. Gastroenterology , DOI: ( /j.gastro ) Copyright © 2004 American Gastroenterological Association Terms and Conditions
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