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Linolenic acid prevents neuronal cell death and paraplegia after transient spinal cord ischemia in rats  Loïc Lang-Lazdunski, MD, PhD, Nicolas Blondeau,

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Presentation on theme: "Linolenic acid prevents neuronal cell death and paraplegia after transient spinal cord ischemia in rats  Loïc Lang-Lazdunski, MD, PhD, Nicolas Blondeau,"— Presentation transcript:

1 Linolenic acid prevents neuronal cell death and paraplegia after transient spinal cord ischemia in rats  Loïc Lang-Lazdunski, MD, PhD, Nicolas Blondeau, PhD, Gisèle Jarretou, BS, Michel Lazdunski, PhD, DSc, Catherine Heurteaux, PhD  Journal of Vascular Surgery  Volume 38, Issue 3, Pages (September 2003) DOI: /S (03)

2 Fig 1 Representative photomicrographs of lumbar spinal cord (L4) sections stained with cresyl violet from rats in sham operation (B, C), ischemic (vehicle; A, D, E), and linolenic acid (LIN; F, G) treatment groups. Except for rats who underwent sham operation, all rats underwent 14 minutes of ischemia and 7 days of reperfusion. Chosen section for illustration of spinal cords in LIN-treated rats is from a rat with LeMay score of 1 that was treated at onset of reperfusion. A, Overview of control spinal cord (×25). B, D, F, High magnification of motor neurons in ventral horn of sham operation, control, and LIN treatment groups, respectively (×100). C, E, G, High magnification of small neurons in dorsal horn of sham operation, control, and LIN treatment groups, respectively (×100). Journal of Vascular Surgery  , DOI: ( /S (03) )

3 Fig 2 Representative photomicrographs of lumbar spinal cord (L4) show morphologic features of apoptosis with TUNEL staining in sections from rats in sham operation (B, C), ischemic (vehicle; A, D, E), and linolenic acid (LIN; F,G) treatment groups. Except for rats in the sham operation group, all rats underwent 14 minutes of ischemia and 24 hours of reperfusion. Chosen section for illustration of spinal cords in LIN-treated rats is from a rat with LeMay score of 1 that was treated at onset of reperfusion. A, Overview of ischemic spinal cord (×25) shows numerous TUNEL-positive neurons scattered within gray matter (dorsal horn, intermediate gray matter, ventral horn). There are also some TUNEL-positive motor neurons. B, D, F, High magnification of motor neurons in ventral horn of sham operation, control, and LIN treatment group, respectively (×100). C, E, G, High magnification of small neurons in dorsal horn of sham operation, ischemic, and LIN treatment groups, respectively (×100). F, G, Treatment with linolenic acid resulted in a dramatic decrease in TUNEL labeling in almost the entire gray matter, especially the intermediate zone and ventral horn. Journal of Vascular Surgery  , DOI: ( /S (03) )

4 Fig 3 Immunohistochemical expression of Bax protein in lumbar spinal cord (L4) sections from rats in sham operation (B, C), ischemic (vehicle; A, D, E), and linolenic acid (LIN; F, G) treatment groups. Except for rats in the sham operation group, all rats underwent 14 minutes of ischemia and 24 hours of reperfusion. Chosen section for illustration of spinal cords in LIN-treated rats is from a rat with LeMay score of 1 that was treated at onset of reperfusion. A, Overview of Bax immunoreactivity in ishemic spinal cord (×25). B, C, There was no Bax expression in ventral and dorsal horns of rats in the sham operation group. A, D, E, After ischemia, intense Bax immunostaining was observed in dorsal and ventral horn neurons. F, G, Spinal cords of LIN-treated rats exhibited complete loss of Bax immunoreactivity in dorsal and ventral horns (×100 magnification). Spinal cord frozen sections were prepared and immunostained with polyclonal Bax antibody (see Material and Methods). Data are representative of four separate experiments in each group (n = 5). Journal of Vascular Surgery  , DOI: ( /S (03) )

5 Fig 4 Immunohistochemical expression of HSP70 in lumbar spinal cord (L4) sections from rats in sham operation (B, C, D), ischemic (vehicle; A, E, F, G), and linolenic acid (LIN; H, I, J) treatment groups. Except for rats in the sham operation group, all rats underwent 14 minutes of ischemia and 24 hours of reperfusion. Chosen section for illustration of spinal cords in LIN-treated rats is from a rat with LeMay score of 1 that was treated at onset of reperfusion. A, Overview of HSP70 immunoreactivity in ischemic spinal cord (×25). Ischemia induced HSP70 immunoreactivity in numerous neurons scattered in the gray matter of dorsal horns (G) and ventral horns (E), but not in the nucleus of motor neurons of control rats (F). H, I, In contrast, linolenic acid induced strong immunoreactivity for HSP70 in the nucleus of motor neurons. Magnification ×100 for B, D, E, G, H and J, C, F, I corresponds to respective photomicrographs B, E, H at higher magnification (×500). Spinal cord frozen sections were prepared and immunostained with polyclonal HSP70 antibody (see Material and Methods). Data are representative of four separate experiments in each group (n = 5). Journal of Vascular Surgery  , DOI: ( /S (03) )

6 Fig 5 Immunohistochemical expression of NF-κB protein in lumbar spinal cord (L4) sections from rats in sham operation (B, C), ischemic (vehicle; A, D, E), and linolenic acid (LIN; F, G) treatment groups. Except for rats in the sham operation group, all rats underwent 14 minutes of ischemia and 24 hours of reperfusion. Chosen section for illustration of spinal cords in LIN-treated rats is from a rat with LeMay score of 1 that was treated at onset of reperfusion. A, Overview of NF-κB immunoreactivity in ischemic spinal cord (×25). B, C, There was no NF-κB expression in ventral and dorsal horns of rats in the sham operation group. A, D, E, After ischemia, intense NF-κB immunostaining was observed in dorsal and ventral horn neurons. F, G, Spinal cords of LIN-treated rats exhibited an important decrease in NF-κB immunoreactivity in dorsal and ventral horns (×100). Spinal cord frozen sections were prepared and immunostained with monoclonal antibody of the active form of NF-κB (see Material and Methods). Data are representative of four separate experiments in each group (n = 5). Journal of Vascular Surgery  , DOI: ( /S (03) )

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