1. Genetic Engineering
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2. A Controversial Topic Add your first bullet point here
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3. How Does it Work Many different ways
First- need to get the gene of interest
Ex. Glow gene from jellyfish
Second- need to amplify (make more of) gene of interest
Polymerase Chain Reaction (PCR)

4. Steps continued Third- obtain a plasmid (cloning vector)
-A plasmid is a small loop of DNA in a bacterium
Four- insert gene into plasmid and have bacteria take up plasmid (transformation)
Five- grow bacteria and harvest the gene or protein!

5. Steps!

7. What does all the DNA cutting?
Restriction enzymes
Cut at particular sequences
Cuts gene of interest out and the plasmid at same bases
Sticky ends are what is typically produced!
To seal the two together, you need ligase!
Then you have recombinant DNA