1. GLUTAMINASE - Phylogenetic tree construction
- The amino acid Cys (C) was found to be statistically significant in mesophilic bacteria as compared to the abundance of this amino acid in extremophilic bacteria
- Statistical analysis revealed GRAVY to be high in thermophilic (2.29 fold) and psychrophilic bacteria (3.3 fold) as compare to mesophilic bacteria
- Phylogenetic tree construction
- Grouped the glutaminases into their respective classes:
- Mesophiles
-Thermophiles
- Psychrophiles
- Halophiles
-Sequences representing L-glutaminase from extremophiles were analyzed
-Physico-chemical properties were studied to relate these observed differences to their extremophilic properties
- Multiple sequence alignment revealed the domain/motif for glutaminases
- Domain/ motif consisted of Ser- 74, Lys-77, Asn-126, Lys-268, and Ser-269, which is conserved in all microorganisms
GLUTAMINASE