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HC70AL Research Presentation
Eric Newton
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First gene AT3G47640 Wild Type: 1,409 Mutant: 730
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Second gene AT3G61310 Wild Type: 621 Mutant: 609
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First gene plants genotype
Plant 1: Heterozygous Plant 2: WT Plant 3: WT Plant 4: Mutant Plant 5: WT Plant 6: WT Plant 7: WT Plant 8: -undetermined- Plant 9: Mutant Plant 10: WT Plant 11: Mutant Plant 12: -undetermined-
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Gene 1 mutant plant phenotype
Why no phenotype for 2nd gene?
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Second gene plants genotype
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Salk vs. my results Gene anatomy according to Salk online database Gene anatomy according to my lab results
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When and where are the genes expressed?
Second Gene First Gene
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Promoter region 1 What were the sequence results? 1-kb Ladder Clone #1
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Promoter region gene 2 What were the sequence results?
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Conclusion Gene 1 -found mutant -no visible phenotype -Promoter cloning obsolete Gene 2 -no found mutant -phenotype N/A -Promoter cloning has mutations
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