1. BioBricks

2. Part Types Chassis – organism (E. coli, yeast, mammalian)
Vectors – plasmids, strains
Parts - Proteins, RBS
Devices – Inverters, reporters

3. Chassis Constructing systems in bacteria (E. coli)
This chassis can interact with most other organisms
Conjugation, bacteriophage, signalling etc.

4. Vectors
Plasmid – circle of extra chromosomal DNA that can be inserted into cells
These can contain biobrick pieces or be a “backbone” into which one can place biobrick pieces
Strains?

5. Parts Protein Coding Regulatory Ribosome Binding Sites Terminators RNA
Conjugation
Regulatory
Terminators
RNA
DNA

6. Regulatory

7. Terminators

8. RNA
Nature Biotechnology 22, (2004)

9. DNA
Spacers
Add Biobricks site
Insertion
Transpososome

10. Protein coding Genes that code for useful proteins
Repressors, activators
Integrases, Excisionases
Enzymes
Reporters

11. Ribosome binding sites
Site where the ribosome binds (obviously)
Different sequences lead to change in translation
These sites can be used for riboregulators or other regulation

12. Conjugation
Genes and plasmids for the transfer of genetic material via conjugation.
kentsimmons.uwinnipeg.ca/16cm05/1116/16monera.htm

13. Devices Protein Generator Reporters Composite Devices Inverters
Signalling
Protein Generator
Composite Devices
Measurement

14. Reporters
Semi-rudundant to DNA as there are genes of reporters in this category
Also reporters expressed by constitutive and regulated promoters
Some more complex systems using reporters

15. Inverters, Logic devices
PoPs -Polymerase Per Second (or PoPS) is defined as the number of times that an RNA polymerase molecule passes a specific point on DNA per unit time

16. Signalling Communication between an individual cell and its neighbors
Signal Senders
Signal Receivers
devices using signalling

17. Protein Generation RBS-gene-terminator promoter-RBS-gene-terminator
operator-promoter-RBS-gene-terminator

18. Composite devices
Catch all device category

19. Measurement Promoter output measurements RBS characterization
QPI refers to a Quad-Part Inverter: an RBS, repressor protein, terminator and promoter
Screening plasmids: promoter, reporter, cloning site, reporter

20. “Other”
PCR primers
Tags – degradation, binding and restriction sites

21. General info
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