1. HPLC TROUBLE SHOOTING
Dr. Mirza Shahed Baig

2. Troubleshooting Locate the problem by ranking possible causes.
There is no standard troubleshooting procedure.
General Pattern:
Locate the problem by ranking possible causes.
Verify the presence of the most probable cause.
If present – fix the problem, otherwise verify the existence of the next possible cause.
First try to distinguish
System problem or
Method problem

3. HPLC SYSTEM

4. Method vs. System Troubleshooting
System Parameters
Flow stability
Backpressure
Clogging
Detector problems
Injection siutability
Injection volume
Temperature
Method Parameters
Flow rate
Eluent type
Eluent composition pH
pH modifier (type)
Gradient profile